A COMPARATIVE STUDY OF THE IN VITRO ACTIVITIES OF TWO ANTIFUNGAL AGENTS ON CANDIDA ALBICANS

Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 9

Abstract

Candida albicans is the principal cause of candidiasis in women. A comparative study of the in vitro activities of two antifungal agents was carried out using clotrimazole and nystatin. Two hundred and fifteen (215) Candida albicans isolated from high vaginal swabs of different females with symptomatic cases of vaginal candidiasis were confirmed using Germ tube test. The minimal inhibitory concentrations (MIC) of the drugs on yeast isolates were determined by broth dilution susceptibility test and absorbance reading using spectrophotometer after 48 hours incubation at 27oC to obtain IC10 and IC50 of the drugs. The results showed that the treatment of Candida albicans with selected sub-lethal concentrations of nystatin (250 mg/l) and clotrimazole (125 mg/l) respectively can induce a reversion to yeast growth known as drug resistance. Clotrimazole produced more therapeutic effect for the inhibition of some isolates with IC50 between 20.04 mg/ml and 17.42 mg/ml while nystatin produced more therapeutic effect for the inhibition of other isolates with IC50 between 10.73 mg/ml and 16.37mg/ml. This shows that 25% drug is required to inhibit the isolates by the drug of choice. Nystatin and clotrimazole showed different therapeutic effects on the isolates due to fungal, drug or host factors. Therefore, adequate antifungal susceptibility testing should be performed on individual basis to ascertain the drug of choice before prescription.

Authors and Affiliations

Prof. Braide W.

Keywords

Related Articles

EFFECT OF EXERCISE ON DIFFERENTIAL LEUCOCYTE COUNT IN HEALTHY MALES. A COMPARATIVE STUDY ON HEMATOLOGICAL PARAMETERS

Introduction: Exercise on regular basis, is largely done to have maintained body shape together with its effect on other physiological parameters of the body. Exercise increases differential leucocyte count and killing c...

PLASMID PROFILE ANALYSIS AND CURING OF MULTIDRUG RESISTANT BACTERIA ISOLATED FROM HOSPITALS WASTE DUMP SITE IN CALABAR METROPOLIS, NIGERIA

This study investigated the plasmid profile analysis and curing of multidrug resistant bacteria isolated from hospitals waste dump site in Calabar metropolis. Samples collection, identification of bacterial isolates, ant...

DERMATOLOGY IN ANCIENT ROME: 27 B.C.-476 A.D.

Dermatology did not become a medical specialty until the early Nineteenth century. This article demonstrates that Roman physicians acquired a significant body of knowledge about common skin disorders and techniques desig...

ACHYRANTHES ASPERA -A PROMISING MEDICINAL HERB

Achyranthes aspera Linn. (Amaranthaceae family) is an important medicinal plant is found throughout Bangladesh as an annual herb. The medicinal plants are used for treatment of various diseases because of their safety an...

FORMULATION AND EVALUATION OF CIPROFLOXACIN-COCKLE SHELLS DERIVED CALCIUM CARBONATE ARAGONITE NANOPARTICLES PHYSICOCHEMICAL PROPERTIES MEDIATED IN VITRO BACTERICIDAL ACTIVITY IN SALMONELLA TYPHIMURIUM

Since the use of classical antibiotics in the management of resistant bacterial infections requires high dosage and regular administration for a lengthy duration, the enhanced delivery system that will ensure sustained r...

Download PDF file
  • EP ID EP674222
  • DOI -
  • Views 133
  • Downloads 0

How To Cite

Prof. Braide W. (2019). A COMPARATIVE STUDY OF THE IN VITRO ACTIVITIES OF TWO ANTIFUNGAL AGENTS ON CANDIDA ALBICANS. European Journal of Pharmaceutical and Medical Research, 6(9), 79-84. https://europub.co.uk/articles/-A-674222