A comparative study on the estimation of serum Creatinine levels by Jaffe's and Enzymatic methods at different levels of serum Bilirubin
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2017, Vol 4, Issue 3
Abstract
In the present study, based upon their total serum bilirubin values ranging from 0.3-30.8 mg/dl, 47 patients visiting OPD and IPD of HIMS were divided into five groups. Their serum creatinine levels were estimated by the Jaffe’s and the Enzymatic methods. Total serum bilirubin concentration was estimated by the timed endpoint Diazo method. Results obtained during the studies revealed that in all the five groups, estimation of serum creatinine by the Jaffe’s method always gave significantly higher values (p< 0.01) as compared to their estimated values by the Enzymatic method at the identical serum bilirubin concentrations. The differences in the mean values of serum creatinine obtained between the two methods were found to significantly increase with the increase in the serum total bilirubin concentrations. At serum total bilirubin concentration of < 2.0 and >20.0 mg/dl, the mean differences between the Jaffe’s and Enzymatic methods were found to be 0.2206 ± 0.0508 and 0.6786 ± 0.0530 mg/dl respectively, resulting in an approximately three-fold increase in the difference in the estimated serum creatinine concentration by the two methods. The highly significant enhancement in the differences in the estimated values of serum creatinine, between the Jaffe’s and the Enzymatic method, with an increase in serum bilirubin concentrations, are attributed to the negative and positive interference in creatinine estimation caused by lower and higher concentrations of total serum bilirubin respectively.
Authors and Affiliations
Monica Kakkar, Rakesh Kakkar
Keywords
Related Articles
- EP ID EP297368
- DOI 10.18231/2394-6377.2017.0071
- Views 68
- Downloads 0
Role of hemodialysis in management of patients of chronic kidney disease
Introduction: As kidney functions deteriorate, lipid profile abnormality begins. In kidney disease, more lipoprotein synthesis than its degradation results in dyslipidemia, which increases the risk of atherogenesis. The...
Serum gamma glutamyl transferase and hs-CRP levels in patients with type 2 diabetes mellitus
Introduction: Type 2 diabetes mellitus and its complications is an important health problem. As oxidative stress and inflammation appears to play a major role in the pathogenesis of diabetes mellitus and development of i...
Pitfalls of currently practiced approach for teaching medical students: opinions & options
Introduction: During a didactic lecture, information is given to the students by the teacher, where students are passive listeners and the teacher is the narrator. Interaction between the students and the teacher, though...
Effect of sample storage and time delay (delayed processing) on analysis of common clinical biochemical parameters
Introduction: The objective of the study was to studying the effect of delay in time on biochemical estimations, the effect of ambient temperature on assays and the effect of hemolysis on certain commonly requisitioned p...
Plasma ascorbic acid levels in patients with subtypes of cataract
Objective: Cataract is a vision impairing disease characterized by gradual, progressive thickening of the lens. It is one of the leading causes of blindness in the world today. Dietary antioxidant vitamins, in particular...