A NEW MODEL FOR INFLAMMATORY BOWEL DISEASE IN RATS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 2
Abstract
Here we report the Lipopolysaccharide (LPS) induced rat model of Inflammatory Bowel Disease (IBD). Toll Like Receptor-4 (TLR-4) are reported to be upregulated in IBD and LPS is recognized by TLRs of the innate immune system. Hence the effect of LPS was investigated for induction of IBD in rats. In the present study, Colitis in male albino wistar rats was developed with different doses of LPS obtained from E.Coli (50, 100, 200 µg/rat, intrarectally) administered on 1st and 5th day. The inflammation produced was evaluated on 7th, 12th and 15th day by comparing with normal as well as 2, 4 dinitrobenzene sulphonic acid (DNBS; 120 mg/kg, intrarectally single dose) treated rats. At the end of experiment, colonic mucosal damage index (CMDI), tissue levels of malondialdehyde (MDA), nitric oxide (NO) and reduced glutathione (GSH) were measured. Colon tissue levels of MDA and NO were higher and GSH levels were lower at all doses of LPS compared to normal rat. There was also decrease in water intake, food intake, % body weight & increase in colon weight, CMDI in LPS induced rats as compared to normal. However, 100 µg/rat dose seemed to be optimum for producing colon inflammation and the symptoms were most prominent on 12th day of first dosing of LPS. These effects were similar to DNBS treatment, but were less prominent. Therefore, the results of our study suggest that Lipopolysaccharide can produce symptoms like inflammatory bowel disease when applied locally.
Authors and Affiliations
Aditi Bariya , Vinay Darji , Shrikalp Deshpande , Gaurang Shah
Keywords
Related Articles
- EP ID EP97711
- DOI -
- Views 102
- Downloads 0
ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF CALENDULA OFFICINALIS
Calendula officinalis, belonging to the family of Asteraceae, commonly known as English Marigold or Pot Marigold is an aromatic herb which is used in Traditional System of Medicine. It is mainly used because of its vario...
PLURIPOTENT STEM CELLS FOR VASCULAR DISEASE THERAPY
Vascular regeneration for ischemic disease represents an unmet clinical need. The ability to derive and expand homogenous populations of human vascular endothelial cells from either human embryonic stem (hES) cells or hu...
DENDRIMERS AND ITS APPLICATION
A dendrimer can described as a macromolecule characterized by its highly branched 3D structure which provides a high degree of surface functionality and versatility Dendrimers also referred to as the “Polymers of the 21s...
CLINICAL ASSESSMENT OF SUSHRUTOKTA DWADASHAVIDHA PARIKSHA IN MADHUMEHA
Present study was carried out to understand the strength (Rogibala) as well as to know the intensity of disease (rogabala) madhumeha (DM) by adopting Sushrutokta Dwadashavidha Pariksha. Present clinical study reveals tha...
INSECTICIDAL ACTIVITY OF Secamone afzelii (SCHULT) K. SCHUM POWDER IN THE CONTROL OF Stiphilous zeamais (MOTS) (COLEOPTERA: CURCULIONIDAE)
Maize seeds are highly susceptible to beetles attack and every year this insect causes great loss in storage. This study investigated the efficacy of the insecticidal properties of Secamone afzelii plants powder against...