A new reverse phase HPLC method for quick quantificationof all four isomers of natural vitamin-E oil in a single analysis
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2014, Vol 3, Issue 4
Abstract
A simple and fast analytical method for quantification of natural vitamin E isomers by reverse phase High Performance Liquid Chromatography within short time. This method is developed to quantify in four natural isomers of vitamin- E, namely alpha, beta, gamma and delta tocopherol in fine formulations. The four isomers elution was carried out by using simple short column (100 X 4.6 mm) were used. And even mobile phase has used only Acetonitrile and water. The Limit of detection was achieved of isomers are very lower levels like 3 ppm. The method is capable to quantify 10 ppm to 100 % in a sample. The method is reliable robust, accurate and linear.
Authors and Affiliations
N. K. Sarathchandraprakash
Keywords
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- EP ID EP656709
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