A NEW REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE SIMULTANEOUS ESTIMATION OF SERRATIOPEPTIDASE AND DICLOFENAC SODIUM IN BULK AND TABLET DOSAGE FORM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The objective is to study the development of a simple, rapid, specific, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of serratiopeptidase (SER) and diclofenac (DC) sodium in bulk and tablet formulation.Methods: RP-HPLC method was developed for the simultaneous estimation of SER and DC sodium in tablet formulation. The separation was achieved by Kromasil C18 column (250 mm × 4.6 mm, 5 μm particle size) with phosphate buffer pH-7 and o-phosphoric acid:methanol:acetonitrile (5:4:1% v/v/v). Flow rate was maintained at 1 mL/min and UV detection was carried at 270 nm.Result: For RP-HPLC method, the retention time for SER and DC sodium was found to be 3.3833 min and 8.1667 min, respectively. The method was validated for accuracy, precision, and specificity. Linearity for SER and DC sodium was in the range of 5–50 μg/ml.Conclusion: The developed RP-HPLC method is simple, accurate, rapid, sensitive, precise, and economic. Hence, this method can be employed successfully for the estimation of SER and DC sodium in both bulk and tablet dosage forms.
Authors and Affiliations
Manasi Kulkarni B, Anagha Joshi M
Keywords
Related Articles
- EP ID EP607114
- DOI 10.22159/ajpcr.2019.v12i1.28857
- Views 128
- Downloads 0
INSECTICIDAL AND GENOTOXIC POTENTIAL OF ACORUS CALAMUS RHIZOME EXTRACT AGAINST DROSOPHILA MELANOGASTER
ABSTRACTObjectives: Insect pest problems in agriculture have a considerable shift during first decade of 21st century due to global climate changes. Varioussynthetic insecticides were used from last 50 years to overcome...
A DEEP INSIGHT ON DIABETIC NEUROPATHY: THE SILENT COMPLICATION OF DIABETES, WITH INPUTS ON ITS CAUSES, DIAGNOSIS, PATHWAYS, AND TREATMENTS
Background: The incidence of diabetic neuropathy (DNP) is a prominent complication for people suffering from diabetes. DNP is a common complication in patients suffering from diabetes, and it is affecting approximately m...
EFFECT OF CRUDE EXTRACT AND PHYTOSTEROL FRACTION OF FUMARIA OFFICINALIS IN INDUCED HYPERTROPHIC SCAR OF RABBITS
Objective: The present study aimed to evaluate the role of crude extract and phytosterol fraction of Fumaria officinalis in experimentally induced hypertrophic scar in rabbits. Methods: Surgical wounds were performed on...
CEFTRIAXONE-INDUCED PERIORBITAL EDEMA
Cephalosporins are widely prescribed antibiotics for common infections and also used for prophylactic measures. Ceftriaxone is a third-generation cephalosporin which is known to cause hypersensitivity reactions presentin...
THERAPEUTIC POTENTIAL AND IN VITRO ANTHELMINTIC ACTIVITY OF RIDGE GOURD FRUIT
Objective: The objective of the study was to evaluate the therapeutic potential and in vitro anthelmintic activity of ridge gourd fruit (Luffa acutangula) against Indian earthworms. Methods: For anthelmintic activity ag...