A NEW REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE SIMULTANEOUS ESTIMATION OF SERRATIOPEPTIDASE AND DICLOFENAC SODIUM IN BULK AND TABLET DOSAGE FORM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The objective is to study the development of a simple, rapid, specific, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of serratiopeptidase (SER) and diclofenac (DC) sodium in bulk and tablet formulation.Methods: RP-HPLC method was developed for the simultaneous estimation of SER and DC sodium in tablet formulation. The separation was achieved by Kromasil C18 column (250 mm × 4.6 mm, 5 μm particle size) with phosphate buffer pH-7 and o-phosphoric acid:methanol:acetonitrile (5:4:1% v/v/v). Flow rate was maintained at 1 mL/min and UV detection was carried at 270 nm.Result: For RP-HPLC method, the retention time for SER and DC sodium was found to be 3.3833 min and 8.1667 min, respectively. The method was validated for accuracy, precision, and specificity. Linearity for SER and DC sodium was in the range of 5–50 μg/ml.Conclusion: The developed RP-HPLC method is simple, accurate, rapid, sensitive, precise, and economic. Hence, this method can be employed successfully for the estimation of SER and DC sodium in both bulk and tablet dosage forms.
Authors and Affiliations
Manasi Kulkarni B, Anagha Joshi M
P-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS
Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.Methods: After informed consent, 96 Sudanese pregnant wome...
PHYTOCHEMICAL SCREENING OF ARTOCARPUS HIRSUTUS AND ITS ANTIMICROBIAL POTENTIAL
Objective: In the present study, the effort was aimed to evaluate the phytochemical components, antioxidant activity, and antimicrobial activity of Artocarpus hirsutus fruit and leaf. Methods: Each plant materials were e...
EVALUATION EVALUATION OF PHYTOCHEMICAL COMPOUNDS AND ANTIMICROBIAL ACTIVITIES OF HEMIDESMUS INDICUS
Objective: The present study was aimed to investigate the phytochemical screening, antibacterial activities, and identification of bioactive compounds by gas chromatography-mass spectrometry (GC-MS) and high-performance...
MELOXICAM-PECTIN-β-CYCLODEXTRIN TERNARY COMPLEX BY KNEADING FOR ENHANCEMENT OF SOLUBILITY AND DISSOLUTION RATE
Objective: The objective of the present investigation was to improve the solubility and dissolution rate of poorly soluble drug meloxicam by its ternary inclusion complexation with natural polymers and beta-cyclodextrin...
MIMOSINE - A NOVEL SUBSTANCE AND EVALUATE THE LIPID PEROXIDATION
Objective: The mimosine is a novel substance extracted and purified from Mimosa pudica. The present study is to evaluate lipid peroxidation (LPO). Methods: The sox halation is used to differentiate extracts. Mimosine is...