A novel 3D printing method for cell alignment and differentiation
Journal Title: International Journal of Bioprinting - Year 2015, Vol 1, Issue 1
Abstract
The application of bioprinting allows precision deposition of biological materials for bioengineering applications. Here we propose a 2 stage methodology for bioprinting using a back pressure-driven, automated robotic dispensing system. This apparatus can prepare topographic guidance features for cell orientation and then bioprint cells directly onto them. Topographic guidance features generate cues that influence adhered cell morphology and phenotype. The robotic dispensing system was modified to include a sharpened stylus that etched on a polystyrene surface. The same computer-aided design (CAD) software was used for both precision control of etching and bioink deposition. Various etched groove patterns such as linear, concentric circles, and sinusoidal wave patterns were possible. Fibroblasts and mesenchymal stem cells (MSC) were able to sense the grooves, as shown by their elongation and orientation in the direction of the features. The orientated MSCs displayed indications of lineage commitment as detected by fluorescence-activated cell sorting (FACS) analysis. A 2% gelatin bioink was then used to dispense cells onto the etched features using identical, programmed co-ordinates. The bioink allows the cells to contact sense the pattern while containing their deposition within the printed pattern.
Authors and Affiliations
Ramya Bhuthalingam, Pei Qi Lim, Scott A Irvine, Animesh Agrawal, Priyadarshini S Mhaisalkar, Jia An, Chee Kai Chua, Subbu Venkatraman
Keywords
Related Articles
- EP ID EP678634
- DOI -
- Views 215
- Downloads 0
Digital Light Processing Based Three-dimensional Printing for Medical Applications
An additive manufacturing technology based on projection light, digital light processing (DLP), three-dimensional (3D) printing, has been widely applied in the field of medical products production and development. The pr...
Additive manufacturing of bone scaffolds
Additive manufacturing (AM) can obtain not only customized external shape but also porous internal structure for scaffolds, both of which are of great importance for repairing large segmental bone defects. The scaffold f...
Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture
Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far. Bioprinting allows precise placement of viable cells in a defined matr...
Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering
Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be direct...
The future of skin toxicology testing – Three-dimensional bioprinting meets microfluidics
Over the years, the field of toxicology testing has evolved tremendously from the use of animal models to the adaptation of in vitro testing models. In this perspective article, we aim to bridge the gap between the regul...