Investigation of process parameters of electrohydro-dynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries
Journal Title: International Journal of Bioprinting - Year 2016, Vol 2, Issue 1
Abstract
Tissue engineering is a promising technology in the field of regenerative medicine with its potential to create tissues de novo. Though there has been a good progress in this field so far, there still exists the challenge of providing a 3D micro-architecture to the artificial tissue construct, to mimic the native cell or tissue environment. Both 3D printing and 3D bioprinting are looked upon as an excellent solution due to their capabilities of mimicking the native tissue architecture layer-by-layer with high precision and appreciable resolution. Electrohydrodynamic jetting (E-jetting) is one type of 3D printing, in which, a high electric voltage is applied between the extruding nozzle and the substrate in order to print highly controlled fibres. In this study, an E-jetting system was developed in-house for the purpose of 3D printing of fibrous scaffolds. The effect of various E-jetting parameters, namely the supply voltage, solution concentration, nozzle-to-substrate distance, stage (printing) speed and solution dispensing feed rate on the diameter of printed fibres were studied at the first stage. Optimized parameters were then used to print Polycaprolactone (PCL) scaffolds of highly complex geometries, i.e., semi-lunar and spiral geometries, with the aim of demonstrating the flexibility and capability of the system to fabricate complex geometry scaffolds and biomimic the complex 3D micro-architecture of native tissue environment. The spiral geometry is expected to result in better cell migration during cell culture and tissue maturation.
Authors and Affiliations
Hui Wang, Sanjairaj Vijayavenkataraman, Yang Wu, Zhen Shu, Jie Sun1 and Jerry Fuh Ying Hsi
Creation of a vascular system for organ manufacturing
The creation of a vascular system is considered to be the main object for complex organ manufacturing. In this short review, we demonstrate two approaches to generate a branched vascular system which can be printed using...
Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer: A parametric study
For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bioprinting. Often, a thin metal layer is applied as laser-absorbing material called dynamic release layer (DRL)....
Hydrolytic Expansion Induces Corrosion Propagation for Increased Fe Biodegradation
Fe is regarded as a promising bone implant material due to inherent degradability and high mechanical strength, but its degradation rate is too slow to match the healing rate of bone. In this work, hydrolytic expansion w...
A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requireme...
Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization
The placenta is a transient organ, essential for development and survival of the unborn fetus. It interfaces the body of the pregnant woman with the unborn child and secures transport of endogenous and exogenous substanc...