Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering
Journal Title: International Journal of Bioprinting - Year 2019, Vol 5, Issue 2
Abstract
Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin (Alg-Gel) hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds with 12 × 12 × 1 mm3 in overall dimensions are physically characterized, and the glass dissolution from PCL/glass composite over a period of 28 days is studied. Alg-Gel composite hydrogel is used as a bioink to suspend ASCs, and scaffolds are then bioprinted in different configurations: Bioink only, PCL+bioink, and PCL/glass+bioink, to investigate ASC viability. The results indicate the feasibility of the solvent-based bioprinting process to fabricate 3D cellularized scaffolds with more than 80% viability on day 0. The decrease in viability after 7 days due to glass concentration and static culture conditions is discussed. The feasibility of modifying Alg-Gel with 13-93B3 glass for bioprinting is also investigated, and the results are discussed.
Authors and Affiliations
Krishna C. R. Kolan, Julie A. Semon, Bradley Bromet, Delbert E. Day, Ming C. Leu
Keywords
Related Articles
- EP ID EP678719
- DOI -
- Views 186
- Downloads 0
Revealing emerging science and technology research for dentistry applications of 3D bioprinting
Science and technology (S&T) on three-dimensional (3D) bioprinting is growing at an increasingly accelerated pace; one major challenge represents how to develop new solutions for frequent oral diseases such as periodonta...
Roles of support materials in 3D bioprinting – Present and future
Bioprinting has been introduced as a new technique in tissue engineering for more than a decade. However, characteristics of bioprinted part are still distinct from native human tissue and organ in terms of both shape fi...
Bioprinting of artificial blood vessels
Vascular networks have an important role to play in transporting nutrients, oxygen, metabolic wastes and maintenance of homeostasis. Bioprinting is a promising technology as it is able to fabricate complex, specific mult...
Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW)
3D bioprinting becomes one of the popular approaches in the tissue engineering. In this emerging application, bioink is crucial for fabrication and functionality of constructed tissue. The use of cell spheroids as bioink...
Creation of a vascular system for organ manufacturing
The creation of a vascular system is considered to be the main object for complex organ manufacturing. In this short review, we demonstrate two approaches to generate a branched vascular system which can be printed using...