Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW)
Journal Title: International Journal of Bioprinting - Year 2018, Vol 4, Issue 1
Abstract
3D bioprinting becomes one of the popular approaches in the tissue engineering. In this emerging application, bioink is crucial for fabrication and functionality of constructed tissue. The use of cell spheroids as bioink can enhance the cell-cell interaction and subsequently the growth and differentiation of cells in the 3D printed construct with the minimum amount of other biomaterials. However, the conventional methods of preparing the cell spheroids have several limitations, such as long culture time, low-throughput, and medium modification. In this study, the formation of cell spheroids by SSAW was evaluated both numerically and experimentally in order to overcome the aforementioned limitations. The effects of excitation frequencies on the cell accumulation time, diameter of the formed cell spheroids, and subsequently, the growth and viability of cell spheroids in the culture medium over time were studied. Using the high-frequency (23.8 MHz) excitation, cell accumulation time to the pressure nodes could be reduced in comparison to that of the low-frequency (10.4 MHz) excitation, but in a smaller spheroid size. SSAW excitation at both frequencies does not affect the cell viability up to 7 days, > 90% with no statistical difference compared with the control group. In summary, SSAW can effectively prepare the cell spheroids as bioink for the future 3D bioprinting and various biotechnology applications (e.g., pharmaceutical drug screening and tissue engineering).
Authors and Affiliations
Yannapol Sriphutkiat, Surasak Kasetsirikul, Yufeng Zhou
Keywords
Related Articles
- EP ID EP678680
- DOI -
- Views 170
- Downloads 0
Solvent-based Extrusion 3D Printing for the Fabrication of Tissue Engineering Scaffolds
Three-dimensional (3D) printing has been emerging as a new technology for scaffold fabrication to overcome the problems associated with the undesirable microstructure associated with the use of traditional methods. Solve...
Utilising inkjet printed paraffin wax for cell patterning applications
We describe a method to prepare patterned environments for eukaryotic cells by inkjet printing paraffin wax onto a substrate. This technique bypasses the requirement to create a master mould, typically required with the...
Graphene Oxide Induces Ester Bonds Hydrolysis of Poly-l-lactic Acid Scaffold to Accelerate Degradation
Poly-l-lactic acid (PLLA) possesses good biocompatibility and bioabsorbability as scaffold material, while slow degradation rate limits its application in bone tissue engineering. In this study, graphene oxide (GO) was i...
Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write
This paper describes the direct laser write of a photocurable acrylate-based PolyHIPE (High Internal Phase Emulsion) to produce scaffolds with both macro- and microporosity, and the use of these scaffolds in osteosarcoma...
Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter
Organ printing is a computer-aided additive biofabrication of functional three-dimensional human tissue and organ constructs according to digital model using the tissue spheroids as building blocks. The fundamental biolo...