High-precision three-dimensional inkjet technology for live cell bioprinting
Journal Title: International Journal of Bioprinting - Year 2019, Vol 5, Issue 2
Abstract
In recent years, bioprinting has emerged as a promising technology for the construction of three-dimensional (3D) tissues to be used in regenerative medicine or in vitro screening applications. In the present study, we present the development of an inkjet-based bioprinting system to arrange multiple cells and materials precisely into structurally organized constructs. A novel inkjet printhead has been specially designed for live cell ejection. Droplet formation is powered by piezoelectric membrane vibrations coupled with mixing movements to prevent cell sedimentation at the nozzle. Stable drop-on-demand dispensing and cell viability were validated over an adequately long time to allow the fabrication of 3D tissues. Reliable control of cell number and spatial positioning was demonstrated using two separate suspensions with different cell types printed sequentially. Finally, a process for constructing stratified Mille-Feuille-like 3D structures is proposed by alternately superimposing cell suspensions and hydrogel layers with a controlled vertical resolution. The results show that inkjet technology is effective for both two-dimensional patterning and 3D multilayering and has the potential to facilitate the achievement of live cell bioprinting with an unprecedented level of precision.
Authors and Affiliations
Daisuke Takagi, Waka Lin, Takahiko Matsumoto, Hidekazu Yaginuma, Natsuko Hemmi, Shigeo Hatada, Manabu Seo
Keywords
Related Articles
- EP ID EP678705
- DOI -
- Views 142
- Downloads 0
Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter
Organ printing is a computer-aided additive biofabrication of functional three-dimensional human tissue and organ constructs according to digital model using the tissue spheroids as building blocks. The fundamental biolo...
Of balls, inks and cages: Hybrid biofabrication of 3D tissue analogs
The overarching principle of three-dimensional (3D) bioprinting is the placing of cells or cell clusters in the 3D space to generate a cohesive tissue microarchitecture that comes close to in vivo characteristics. To ach...
The mussel-inspired assisted apatite mineralized on PolyJet material for artificial bone scaffold
With the development of three-dimensional (3D) printing, many commercial 3D printing materials have been applied in the fields of biomedicine and medical. MED610 is a clear, biocompatible PolyJet material that is medical...
Development and characterization of a photocurable alginate bioink for three-dimensional bioprinting
Alginate is a biocompatible material suitable for biomedical applications, which can be processed under mild conditions on irradiation. This paper investigates the preparation and the rheological behavior of different pr...
3D bioprinting processes: A perspective on classification and terminology
This article aims to provide further classification of cell-compatible bioprinting processes and examine the concept of 3D bioprinting within the general technology field of 3D printing. These technologies are categorize...