A Novel Decrease of Mrp3 Protein in Liver of β-Thalassemic Mouse
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 14, Issue 10
Abstract
Background: Hepatocytes have a fundamental system of efflux proteins that protect cells from toxic insults. Unconjugated bilirubin at higher concentration is toxic to cells and its intracellular accumulation is limited by the induction of efflux proteins such as Mrp3. In vivo studies showed an induction of hepatic Mrp3 expression in response to non-hemolytic hyperbilirubinemia as a compensatory mechanism to reduce UCB toxicity. Study Design: In the present study, we analyzed the hepatic Mrp3 expression profile during hemolytic hyperbilirubinemia. We used β-thalassemic mouse and WT rodents treated with phenylhydrazine as an animal model of chronic and acute hemolysis, respectively. Results: Unexpectedly, Mrp3 protein was 75% down-regulated in β-thalassemic mouse although Mrp3 mRNA was normal. Mrp3 mRNA was significantly induced in PHZ treated animals while again; Mrp3 protein was 60% down-regulated. Conclusion: For the first time we observed a clear down-regulation for hepatic Mrp3 protein that linked to hemolysis, not to bilirubin. We hypothesize that a similar decrease for hepatic Mrp3 proteins is occur in hemolytic patients such as β-thalassemia.
Authors and Affiliations
Mohammed Qaisiya, Lucia de Franceschi, Achille Iolascon, Claudio Tiribelli, Cristina Bellarosa
Keywords
Related Articles
- EP ID EP340702
- DOI 10.9734/BJMMR/2016/25139
- Views 74
- Downloads 0
Reproductive Hormonal Profile of Post-menopausal Women in Ebonyi State, Nigeria
Background: Assessment of the concentrations of reproductive hormones during post-menopause has been suggested as a confirmatory test for menopause due to irregularities in rise and fall of sex hormones in menopausal tra...
Prescribing Guide for Baclofen in the Treatment of Alcoholism – for Use by Physicians
The purpose of this guide is to help doctors prescribe baclofen in the treatment of alcohol problems as there is, to date, no standardized way to prescribe this molecule in the treatment of alcohol dependence. The Recomm...
Case Report of Ring Chromosome 13: 46,XX,r(13)(p13q34)/46,XX,dic r(13;13)(p13q34;p13q34)
Aims: To report a case of ring chromosome 13 in a female child. Presentation of Case: Female, Caucasian, born in Southeast of Brazil, 6 years old. Born by cesarean section, the physical examination at 6 years and 1 month...
Unsedated Colonoscopy: Experience from an Endoscopy Centre
Aim and Objective: Colonoscopy is generally considered a painful procedure requiring sedation. Due to the high cost of sedation colonoscopy, coupled with the attendant morbidity and mortality, there is a general trend to...
Differential and Combined Effects of Simvastatin and Vildagliptin on Angiogenic Markers and Oxidative Stress in Hind Limb Model of Ischemia in Diabetic Rats
Background: This study investigated the potential therapeutic effect of simvastatin and vildagliptin and their combination on angiogenesis in diabetic hind-limb ischemia. Methods: 60 Sprague-dawely rats were divided int...