A Novel Gradient RP-HPLC Method Development for Bortezomib in Parenteral Dosage Form
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2013, Vol 2, Issue 2
Abstract
The objective of present study was the development of gradient RP-HPLC method for the estimation of bortezomib in parenteral dosage form. The analysis was performed on Hypersil C18 column (250 x 4.6mm) with mobile phase composed of acetonitrile: water: THF (65:35:5), maintaining flow rate at 1ml/min. with retention time of 3.5±0.5min. The detection was done at 280nm. A regression coefficient was 0.999 obeying the Beer’s law at concentration range of 20 – 120 μg/ml. The proposed method was validated for specificity, linearity, accuracy, precision and robustness as per ICH guidelines and used successfully for bortezomib determination in routinely quality control.
Authors and Affiliations
Akshay Kumar Bisht*| Jaipur Nation University, Jaipur, Rajasthan, India-302025, Brij Bhushan| ASBASJSM College of Pharmacy, BELA, Ropar, Punjab, India-140111, Vandana Dhiman| ASBASJSM College of Pharmacy, BELA, Ropar, Punjab, India-140111, R. K. Dhawan| Khalsa College of Pharmacy, G.T. Road, Amritsar, Punjab, India-143002, U. S. Baghel| Khalsa College of Pharmacy, G.T. Road, Amritsar, Punjab, India-143002, Anil Kumar Gupta| Research Scholar, Bhagwant University, Institute of Pharmacy & Research Center, Ajmer, Rajasthan, India-305004
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