Demonstration the Expression of α-Methyl Acyl-Co EnzymeA Racemase, P63 and high- molecular-weight cytokeratin in Prostate biopsies in Khartoum State Hospitals
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2016, Vol 5, Issue 6
Abstract
Prostate cancer is the major health problem throughout the world. PCa remains the most common malignancy affecting men and the second leading cause of cancer-related death of men. The immunohistochemistry plays a very important role in the diagnosis, but it’s largely depends on panels of markers. Materials and Methods: This is descriptive cross sectional study carried out in Khartoum state hospitals during the period from November 2015 to September 2016. One hundred samples formalin fixed paraffin embedded biopsies were collected including 50 with BPH and 50 with adenocarcinoma of PCa and applied by tissue micro array for IHC staining using p63, high molecular weight cytokeratin(HMWCK) and alpha methyl acyl co A racemace(AMACR) with polymer method. Results: One hundred samples were collected, out of which 43 (86%) of 50 samples benign prostatic hyperplasia and 46 (92%) of 50 samples adenocarcinoma were over the Age 60. Our result show that 33 of 50 samples had G.S 4,6,7 each of them had 11 frequencies 12 of 50 samples had G.S 5, and 5 of 50 samples with G.S 8. In our results we show that in 50 samples of BPH 7, 44 and 40 were positive in AMACR, P63 and HMWCK respectively. Also we found in 50 samples of adenocarcinoma 40, 5 and 8 were positive in AMACR, P63 and HMWCK. Conclusions: immunohistochemistry is helpful and necessary to distinguish prostate cancer from benign mimickers, and we have to use AMACR, P63 and HMWCK in combinations in routine diagnosis.
Authors and Affiliations
Yosra Ibrahim Sid Ahmed Ali| Bsc. Msc. in Histopathology and Cytology, Ahmed M. Hamed, (M.Sc, PhD, MBBS)| Medical Physician and Consultant Clinical Laboratory Scientist;-Assistant Professor of Histopathology and Cytology
Keywords
Related Articles
- EP ID EP3792
- DOI -
- Views 421
- Downloads 20
PHOSPHODIESTERASE: WHAT IS IN AN ENZYME?
Phosphodiesterases (PDEs) are hydrolytic enzymes that degrade intracellular cyclic nucleotides. By altering the concentration of these second messengers, PDEs tend to control cellular and subcellular functioning. PDEs ar...
INFLUENCE OF TYPE AND DURATION OF PSORIASIS ON SERUM TOTAL CHOLESTEROL IN SUDANESE PATIENTS ATTENDING KHARTOUM TEACHING HOSPITAL FOR DERMATOLOGY AND VENEREAL DISEASES
Background: Psoriasis is an inflammatory dermatosis that is characterized with excessive cellular replication. The high prevalence of atherosclerosis has been reported in psoriatic patients. High serum lipid level has b...
Transcriptional R loops and their significance in neurodegenerative disease and possible elimination by specific carrier mediated microRNA techniques; importance for clinical therapeutics
The transcription process has been shown to stimulate both mutation and recombination in many living beings from bacteria to mammals, but the mechanisms underlying this phenomenon are still largely unknown. A particularl...
CARBAZOLE: IT’S BIOLOGICAL ACTIVITY
Carbazole alkaloids constitute an important class of naturally occurring heterocycles, isolated from the Rutaceaefamily. First Carbazole alkaloids were isolated as natural products from Murraya koenigii that exhibited s...
In Vitro Release Kinetics Study of Indomethacin 12Hr Matrix Tablet From Methocel K4M CR and Methocel K15M CR.
The objective of the present study was to design and evaluate controlled release tablets of Indomethacin, employing release retarding materials semi synthetic polymers Methocel K4M CR and Methocel K15M CR. There were nin...