A NOVEL HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF CO-ENZYME Q10 AND α-TOCOPHEROL IN BULK AND PHARMACEUTICAL FORMULATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 10
Abstract
Objective: HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline.Methods: The chromatograms were developed using a mobile phase of Toluene: ethyl acetate: chloroform (10:1:2 v/v/v) on Pre-coated silica 60F 254 plates and quantified by densitometric absorbance mode at 280 nm.Results: The Rf values were 0.77 and 0.87 for co-enzyme Q10 and α-tocopherol, respectively. The linearity of the method was found to be in the concentration range of 0.6µg-1.8 µg/band for α-tocopherol and 2 µg-6 µg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 µg/band for α-tocopherol and 3.441 and 10.42 µg/band for co-enzyme Q10.Conclusion: Developed densitometric method was found to be robust, precise, accurate, and rapid and can be used to analyse fixed-dose capsule samples of co-enzyme Q10 and α-tocopherol.
Authors and Affiliations
Manasi B. Kulkarni, Anagha M. Joshi, Rohini V. Patil
Keywords
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- EP ID EP566142
- DOI 10.22159/ijpps.2018v10i10.28828
- Views 63
- Downloads 0
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