A novel neutral protease from thermophilic Bacillus strain HUTBS62
Journal Title: Journal of BioScience and Biotechnology - Year 2012, Vol 1, Issue 2
Abstract
A novel neutral highly thermostable protease was detected in the culture medium of thermophilic Bacillus strain HUTBS62 isolated from hot-spring located near to the Dead Sea, Jordan. The enzyme was purified by precipitation with 55-60% ammonium sulfate, gel filtration on Sephadex G-100 and DEAE ion exchange chromatography. The enzyme was purified 53-fold with 2% yield. The optimum pH and temperature for catalytic activity of protease was pH 6.8 and 80ºC, respectively, and 31% activity of protease remained even after heat treatment at 100ºC for 60 min. The relative activity of the enzyme was highly stable (90%) at 50ºC for 2 h. The half-life of the enzyme at 90ºC, 80ºC and 70ºC was estimated to be 3, 4 and 6 h, respectively. The activation energy of denaturation of purified enzyme was 21.7 kJmol-1. Iron, sodium, calcium, and manganese increased protease activity. On the other hand, magnesium, cobalt and zinc variably decreased the residual activity. But cadmium and copper drastically inhibited the enzyme activity. The enzymatic activity was highly stable in the presence of 1 and 2 mM EDTA at pH 6.8 and 80ºC. The neutral protease therefore could be defined as a highly thermostable with new properties make the present enzyme applicable for many biotechnological purposes.
Authors and Affiliations
Hazem Aqel, Farouk Al-Quadan, Tahani Yousef
Keywords
Related Articles
- EP ID EP86996
- DOI -
- Views 88
- Downloads 0
Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties
Leuconostoc mesenteroides NRRL B-1149 was evaluated for its probiotic properties. It displayed in vitro cell surface traits in terms of hydrophobicity, autoaggregation property and cell adhesive capacity on HeLa, m...
Horse chestnut (Aesculus hippocastanum L.) as a biomonitor of air pollution in the town of Plovdiv (Bulgaria)
The present study is a small part of a program for application the methods of passive and active phytomonitoring with herbaceous species, trees, mosses and lichens for assessment of the anthropogenic factor in urban cond...
Myostatin gene (MSTN) polymorphism with a negative effect on meat productivity in Dzhalginsky Merino sheep breed
One of the most important negative regulator of muscle grow in mammalians is myostatin. Some mutations in myostatin gene (MSTN) can decrease the effect of protein and play role in meat quality of sheep. Therefore,...
Craniometrical description of striped mouse (Apodemus agrarius Pallas, 1771) in Bulgaria as concerns of discovering of its European population patterns of similarity
Comparative analysis of variations in 50 craniological parameters in 117 adult (59 male and 58 female) individuals of the striped mouse (Apodemus agrarius Pallas, 1771) in Bulgaria that belong to populations with 4...
Recent developments in atom transfer radical polymerization initiators for development of polymer-protein bioconjugates
One of the major challenges in modern synthetic polymer chemistry is to synthesize end defined polymers of different end functionality with predetermined uniform molecular weight. End functionalized polymers/copolymers b...