A NOVEL RP-HPLC METHOD FOR THE QUANTIFICATION OF RUXOLITINIB IN FORMULATIONS
Journal Title: Journal of Atoms and Molecules - Year 2012, Vol 2, Issue 2
Abstract
A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Ruxolitinib in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5μm in particle size) at ambient temperature. The mobile phase consisted of Acetonitrile: Water: Tetra Hydro Furan (THF) 60:30:10% (V/V/V). The UV detection wavelength was 227nm and 20μl sample was injected. The retention time for Ruxolitinib was 4.28min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Ruxolitinib in tablet dosage form and bulk drug.
Authors and Affiliations
P. V. V. Satyanarayana, Alavala Siva Madhavi
Keywords
Related Articles
- EP ID EP367595
- DOI -
- Views 107
- Downloads 0
EXTRACELLULAR BIO-SYNTHESIS OF GOLD NANOPARTICLES USING Escherichia coli AND DECIPHERING THE ROLE OF LACTATE-DEHYDROGENASE USING LDH KNOCK OUT E. coli
This paper is devoted to apprehend the exploitation of biological machinery and optimum biosynthesis parameters using Escherichia coli exudates for gold nanoparticle (GNP) synthesis and its modus operandi. The most influ...
PHYTO CHEMICAL SCREENING AND ISOLATION OF COUMARIN COMPOUNDS FROM MEDICINAL PLANT VACHA BARK
Vacha plant is medicinally useful plant. In order our phytochemical screening we identified a coumarins in Vacha plant. We are extracted and isolated coumarin and identified a compound in the Vacha bark.
QUALITATIVE AND QUANTITATIVE ANALYSIS OF AUREOMYCIN IN MILK AND DAIRY PRODUCTS.
Aureomycin (or-e-o-maisin) is a yellow crystalline Antibiotic and used to treat certain bacterial and Rickettsial Diseases. It is commonly known as chlortetracycline. This Antibiotic has the chemical substance derived fr...
ISOTHERMAL KINETIC AND THERMODYNAMIC STUDIES ON BASIC DYES SORPTION USING RICE HUSK CARBON
Dyes are usually present in trace quantities in the treated effluents of many industries. The present study investigates the potential use of pretreated rice husk and rice husk ash for the removal of methylene blue from...
IDENTIFICATION OF PHENOLIC COMPOUNDS IN VARIOUS MEDICINAL PLANTS
Phenolic compounds make up one of the major families of secondary metabolites in plants and they represent a diverse group of compounds. Phenolics can be broadly divided into non-soluble compounds such as condensed tanni...