A NOVEL STABILITY INDICATING RP-HPLC METHOD FOR THE DETERMINATION OF KETOROLAC TROMETHAMINE IN PHARMACEUTICAL FORMULATIONS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2015, Vol 8, Issue 2
Abstract
 Objective: The main objective of the present investigation was to develop a simple isocratic stability indicating reversed phase liquid chromatographicmethod and validate the proposed method and to apply it for the estimation of ketorolac tromethamine, a non-steroidal anti-inflammatory drug inpure and pharmaceutical formulations.Methods: Waters high performance liquid chromatographic 2695 series system, inertsil ODS, C18 (150 mm × 4.6 mm × 5.0 μ) column and UV-visibledetector with photo diode array detection was adopted for the method development. The components were separated by injecting about 20 μLworking standard solution of concentration 5 μg/mL, using mobile phase prepared by mixing buffer of 100 mM potassium dihydrogen orthophosphatesolution of pH 4.5 and acetonitrile in the ratio 60:40 v/v at a flow rate of 0.8 mL/minute, and the components were detected at a wavelength of 316 nm.Results: The proposed method was validated as per ICH guidelines. The developed method was found to be precise, accurate, linear, robust andrugged. A study of forced degradation was carried out and found that the drug was stable under variety of degradation conditions.Conclusion: The proposed method was adopted for assay of pharmaceutical formulations and recommended for routine analysis in any qualitycontrol laboratory.Keywords: Ketorolac tromethamine, Validation, Assay, Forced degradation, Pharmaceutical formulations.
Authors and Affiliations
Chintala Rambabu, Bandala Koteswara, Golkonda Ramu, Illuru Jyothsna Kumari
Keywords
Related Articles
- EP ID EP605702
- DOI -
- Views 73
- Downloads 0
SYNTHESIS OF SOME NOVEL (E)-METHYL 2,4-DIMETHYL-5-(3-OXO-3-PHENYLPROP-1-EN-1- YL)-1H-PYRROLE-3-CARBOXYLATE DERIVATIVES AS ANTIMICROBIAL AGENT
Objective: The objective of the present study was to synthesize a series of some novel (E)-methyl 2,4-dimethyl-5-(3-oxo-3-phenylprop-1-en-1-yl)-1H-pyrrole-3-carboxylate derivatives and to evaluate it’s in vitro antimicro...
A RANDOMIZED CLINICAL ENDPOINT STUDY TO EVALUATE THE SAFETY AND EFFICACY OF CLEARLIV TABLETS IN PATIENTS WITH ALCOHOLIC LIVER DISEASE
Objective: The objective of this study was to evaluate and compare the hepatoprotective effect of clearliv tablets with silymarin in patients with alcoholic liver disease.Methods: This was a prospective, randomized, mult...
AN OVERVIEW ON DRUG-INDUCED HEPATOTOXICITY
Liver is a vital organ, contributing in most of the metabolic and physiological processes of our body. It plays principal role in detoxification of various drugs and xenobiotics. Though liver possess tremendous regenerat...
CHARACTERISTICS OF PATIENTS WITH ORGANOPHOSPHORUS POISONING AND ITS MANAGEMENT: PHARMACOEPIDEMIOLOGICAL EVALUATION
Objective: The objective of this study was to analyze demographics, management pattern, and clinical outcomes in patients with organophosphorus poisoning. Methods: In this subgroup analysis of retrospective data of pati...
BINARY QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP ANALYSIS IN RETROSPECTIVE STRUCTURE-BASED VIRTUAL SCREENING CAMPAIGNS TARGETING ESTROGEN RECEPTOR ALPHA
  Objective: The objective of this study is to construct predictive unbiased structure-based virtual screening (SBVS) protocols to identify potent ligands for estrogen receptor alpha by combining molecular docking, pro...