A Porcine Model for Repair of Long Bone Non-Union Defects Using Three-Dimensionally Printed Scaffolds
Journal Title: Journal of Regenerative Medicine & Biology Research - Year 2021, Vol 2, Issue 2
Abstract
Background: Approximately 5-10% of bone injuries in the United States result in non-union, costing an estimated $10.4-26.1 billion annually for treatment. While several orthopedic strategies to address non-unions in long bones are available, all have drawbacks and risks of complications. A possible alternative is the recent advances in tissue engineering treatments that are improvements over current treatment options. Methods and Findings: We have developed a porcine long bone segmental defect model for testing osseous non-union repair using 3D-printed biodegradable scaffolds. We generated a 3.5 cm mid-diaphyseal defect in the right radius of a six-month-old pig. A Polycaprolactone (PCL) scaffold implant designed from radius Computed Tomography (CT) scans and produced using solid form fabrication was implanted into the defect and stabilized with a stainless-steel plate. The animal could stand and place weight on the limb at six hours post-surgery and ambulating without a noticeable limp by four weeks. CT imaging post-surgery show bone fill in the defect space by two weeks, significant defect closure by eight weeks and complete union by 24 weeks. This healing has taken place on a PCL scaffold with no other treatments in conjunction with the implant. Conclusion: The porcine radius segmental defect model provides a viable platform for testing printed scaffolds and bioengineered composite scaffold constructs for the efficacy of healing segmental defects and other forms of non-union in long bones.
Authors and Affiliations
Derek J Milner, Sierra A Long, Colleen L Flanagan, Scott J Hollister, Robert Gurtler, Robert Bane, Jerrad Zimmerman, Jo Ann Cameron, Santiago D Gutierrez-Nibeyro, Matthew B Wheeler
Keywords
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- EP ID EP698856
- DOI http://dx.doi.org/10.46889/JRMBR.2021.2203
- Views 49
- Downloads 0
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