A rapid and simple strategy for the formation and isolation of astaxanthin high-yielding Xanthophyllomyces dendrorhous mutant variants
Journal Title: International Journal of Engineering and Science Invention - Year 2018, Vol 7, Issue 7
Abstract
To obtain the positive mutant Xanthophyllomyces dendrorhous strains producing the astaxanthin, a novel breeding strategy of atmospheric and room temperature plasma (ARTP) mutagenesis with 2-deoxyglucose resistance screening was developed. After three successful rounds of recursive breeding, one strain designated B-2m-8 was selected, which was elevated by 187.14 % compared to that of the parent strain 5-7. This work provided an appropriate strategy for obtaining high astaxanthin-yield strains by a novel random mutation method with an efficient screening assay. The results of mutation method and resistance screening indicated that ARTP combined with 2-deoxyglucose resistance was an efficient breeding approach for the rapid evolution of astaxanthin-producing Xanthophyllomyces dendrorhous strains. Finally, the prospect of ARTP mutagenesis is discussed.
Authors and Affiliations
Min Kong, Wen Xu, Hongpeng Wang, Hui Tang, Liping Zhang
Keywords
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