A RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF EPLERENONE IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: To develop a rapid, sensitive, accurate, precise, linear and rugged Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Eplerenone in tablets.Methods: The optimized method uses a reverse phase column, Waters Symmetry C18 (250 X 4.6 mm; 5μ), a mobile phase of triethylammonium phosphate buffer (pH 2.3):acetonitrile in the proportion of 40:60 v/v, flow rate of 1.0 ml/min and a detection wavelength of 240 nm using a UV detector.Results: The developed method resulted in Eplerenone eluting at 3.63 min. Eplerenone exhibited linearity in the range 15-45μg/ml. The precision is exemplified by relative standard deviation of 0.34%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) was found to be 39.16μg/ml and 118.66μg/ml respectively.Conclusion: A sensitive, rapid, accurate, precise, linear and rugged RP-HPLC method was developed and validated for the quantitative estimation of Eplerenone in tablets as per ICH guidelines and hence it can be used for routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
D. Vijay Kumar, S. Srinivas, A. Ashok Kumar
DEVELOPMENT AND VALIDATION OF A BIOANALYTICAL RP-HPLC METHOD FOR AZILSARTAN MEDOXOMIL WITH LIQUID-LIQUID EXTRACTION
Objective: There are many analytical methods available for estimation of Azilsartan medoxomil in biological samples and for pharmaceutical preparations. However, no specific RP-HPLC method with UV detection based on liqu...
NATURAL PRODUCTS AS AN IMPORTANT LEADS FOR DISCOVERY OF NEW ANTITUBERCULAR AGENTS: A REVIEW
There is a very much need for a discovery of new molecules a potent molecule that can cure tuberculosis and prevent the recurrence. A multidisciplinary approach is required to procure a potent bioactive compound and this...
IMPROVING ANTIBIOTIC PRESCRIBING PATTERN AND ASSESSMENT OF CO-MORBIDITIES ASSOCIATED WITH RESPIRATORY TRACT INFECTIONS
Objective: The objective of this study was to describe the antibiotic prescribing pattern and co-morbidities associated with lower respiratory tract infections by means of cross-observational study.Methods: A cross-secti...
SILYMARIN ELICITS PARTIAL PROTECTION AGAINST METHOTREXATE-INDUCED HEPATOTOXICITY IN WISTAR RATS
Objective:Â Methotrexate (MTX) is the gold standard in rheumatoid arthritis treatment; however, its continued use is related to hepatotoxicity. Thus, the aim of the present work is to evaluate the protective role of sil...
AMELIORATIVE ROLE OF BEE HONEY AND ROYAL JELLY AGAINST CISPLATIN INDUCED ALTERATION IN HEMATOLOGICAL PARAMETERS IN MALE WISTER ALBINO RAT
Objective: This study aims to investigate the ameliorative role of dietary bee honey and royal jelly against cisplatin-induced alterations in hematological parameters in male wistar albino rat.Methods: Male wistar albino...