A RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF INDINAVIR IN CAPSULES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Indinavir sulphate (400mg) in capsules.Methods: The optimized method uses a reverse phase column, Enable Make C18G (250 X 4.6 mm; 5μ), a mobile phase of triethylammonium phosphate buffer (pH 2.5): acetonitrile in the proportion of 50:50 v/v, flow rate of 1.0 ml/min and a detection wavelength of 220 nm using a UV detector.Results: The developed method resulted in Indinavir sulphate eluting at 2.7 min. Indinavir sulphate exhibited linearity in the range 50-150μg/ml. The precision is exemplified by relative standard deviation of 1.7%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) was found to be 4.34ng/ml and 13.15ng/ml respectively.Conclusion: An accurate, precise and linear RP-HPLC method was developed and validated for the quantitative estimation of Indinavir sulphate in INDIVAN (400mg) capsules as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
Rajitha Kothapelly, Lakshmi Prasanna Nalluri, Naveen Kumar Rachawar, Ranjith Ch, Ashok Kumar Akuthota
Keywords
Related Articles
- EP ID EP579750
- DOI -
- Views 83
- Downloads 0
EFFECT OF A PARTICLE ENGINEERING PROCESS ON POLYMORPHIC TRANSITION OF PYRAZINAMIDE
Objective: The objective of this work was to investigate the effect of processing on physicochemical properties of spray dried Pyrazinamide. Methods: Pyrazinamide was spray dried using several spray drying conditions, FT...
TOPICAL BENZOPHENONE-3 MICROEMULSION-BASED GELS: PREPARATION, EVALUATION AND DETERMINATION OF MICROBIOLOGICAL UV BLOCKING ACTIVITY
Objective: Microemulsions (MEs) have been developed as active vehicles for sunscreens being physical sunscreens on their own.The aim of this study is to incorporate Benzophenone-3 (BZ-3), a chemical sunscreening agent in...
SIMPLE, EFFICIENT AND ECONOMIC METHOD FORISOLATION AND ANALYSIS OF KARANJIN AND PONGAMOL FROM KARANJA SEED OIL AND SCREENING OF ANTIMICROBIAL POTENTIAL
Objective: Study was undertaken with the objective, to develop simple, high yielding and economic method for the isolation as well as analysis of Karanjin and Pongamol from Karanja oil.Methods: Karanja oil was subjected...
Phytotherapeutic control of food borne pathogens by Jasminum sambac L. flowers
Objective: This study is aimed to determine the antibacterial effect of Jasminum sambac against foodborne pathogens.Methods: Antibacterial activity of methanol and chloroform extract of J. sambac flowers against foodborn...
EFFECTS OF ETHANOL ON MOUSE EMBRYONIC STEM CELL DIFFERENTIATION
Objective: Chronic alcohol consumption during gestation causes fetal malformations, termed fetal alcohol syndrome (FAS). We conducted the present study to clarify the mechanism underlying alcohol consumption-induced malf...