A RAPID STABILITY INDICATING HPLC-METHOD FOR DETERMINATION OF NORETHISTERONE ACETATE IN PLASMA, TABLETS AND IN A MIXTURE WITH OTHER STEROIDS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objectives: This study reports about a simple, rapid and accurate, routine-HPLC method for quantification of Norethisterone acetate (NA) in bulk powder, tablets and plasma.Methods: The chromatographic separation is carried out using isocratic binary mobile phase consisting of acetonitrile (ACN) and water (H2O) in the ratio of 55: 45 at the flow rate of 1 mLmin-1 and 40 °C. A diode array detector is used at 240 nm for detection.Results: The elution time of NA is found to be 2.946±0.01 minutes. The method is validated for system suitability, linearity, precision, limits of detection and quantitation, specificity, stability and robustness. The robustness study is done for small changes in temperature, flow rate, the wavelength of detection, mobile phase content of ACN and injection volume. Stability tests are done through exposure of the analyte solution for four different stress conditions: Reflux with 1N hydrochloric acid (HCl), reflux with 1N sodium hydroxide (NaOH), reflux with 30% hydrogen peroxide (H2O2) and exposure to ultraviolet radiation (UV) radiation. The limits of detection and quantitation are 0.0625 and 0.125 µgmL-1, respectively. The recovery value of this method is 101.90% and the reproducibility is within 2.16.Conclusion: This is a rapid stability indicating HPLC method for determination of NA and can be applied for determination of NA in presence of other steroids as Levonorgestrel, Estradiol and Norethisterone.Â
Authors and Affiliations
Hisham Hashem, Soad Abd El-hay, Thomas Jira
Keywords
Related Articles
- EP ID EP579078
- DOI -
- Views 48
- Downloads 0
EVALUATION OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF FENUGREEK (TRIGONELLA FOENUM-GRAECUM) EXTRACTS
Objective: The present investigation was performed to evaluate the antimicrobial potential of Trigonella foenum-graecum leaves extracts and to elucidate the presence of phytochemicals responsible for its biological activ...
EVIDENCES FOR THE PROMISING THERAPEUTIC POTENTIAL OF BOSWELLIA SERRATA AGAINST ALZHEIMER'S DISEASE: PRE-CLINICAL STUDY
Objective: The current study was planned to investigate the anti-inflammatory and antiapoptotic effects of Boswellia serrata methanolic extract against neurodegeneration characterizing Alzheimer’s disease (AD) in rat m...
A QUALITATIVE STUDY EXPLORING THE IMPACT OF STIGMA IN THE LIVES OF ADULT ASTHMA PATIENTS IN SELANGOR MALAYSIA
Objective: This study aimed to address and explore the issue of stigmatization and its dynamics in asthma patients from hospital and community pharmacy settings.Methods: The qualitative study approach was used to explore...
ANALGESIC EFFECT AND ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS EXTRACT OF BOSWELLIA DALZIELII (BURSERACEAE) STEM BARK
Objective: To evaluate the analgesic effect and anti-inflammatory properties of Boswellia dalzielii (Burseraceae), a medicinal plant commonly used in northern Nigeria as an anti-diarrhoeal, antipyretic, analgesic and ant...
ENHANCING ORAL BIOAVAILABILITY OF CARVEDILOL USING SOLID DISPERSION TECHNIQUE
Objective: Carvedilol (CRV) is a beta blocker drug that is basically used for the treatment of hypertension, heart failure and arrhythmias. The objective of this study is to increase the oral bioavailability of CRV by us...