A standardized research protocol for platelet-rich plasma (PRP) preparation in rats
Journal Title: RSBO - Year 2011, Vol 8, Issue 3
Abstract
Introduction: The urgent need for studies using standardized protocols to evaluate the real biological effects of PRP has been emphasized by several authors. Objective: The purpose of this study was to standardize a methodology for autologous Platelet-Rich Plasma (PRP) preparation in rats. Material and methods: Twenty-four, 5 to 6-month-old, male rats, weighing 450 to 500 g were used. After general anesthesia, 3.15 ml of blood was collected from each animal, via cannulation of the jugular vein. A standardized technique of double centrifugation was used to prepare PRP. PRP samples and peripheral blood platelets were then manually counted using a Neubauer chamber. Student’s t-test was used to compare the differences between the number of platelets in peripheral blood and PRP samples (p < 0.05). In addition, PRP and peripheral blood smears were stained to see platelets’ morphology. Results: All surgical procedures were well tolerated by the animals and theywere healthy during the entire experimental period. PRP samples showed higher significantly platelet concentrations than peripheral blood samples (2,677,583 and 683,680 respectively). Conclusion: Within the limits of this study, it can be concluded that the method used produced autologous PRP with appropriated platelet quantity and quality, in rats. were healthy during the entire experimental period. PRP samples showed higher significantly platelet concentrations than peripheral blood samples (2,677,583 and 683,680 respectively). Conclusion: Within the limits of this study, it can be concluded that the method used produced autologous PRP with appropriated platelet quantity and quality, in rats.
Authors and Affiliations
Michel Messora, Maria Nagata, Flávia Furlaneto, Rita Dornelles, Suely Bomfim, Tatiana Deliberador, Valdir Garcia, Alvaro Bosco
Keywords
Related Articles
- EP ID EP85780
- DOI -
- Views 233
- Downloads 0
Evaluated the incidence of dental fluoroses in school children the public net education in Joinville
This study evaluated the incidence of dental fluorosis in 640 six to twelve year-old schoolchildren of the public education net. The data allow the delimitation and establishment of community and individual fluorosis rat...
A Influência de Diferentes Métodos de Obtenção NA Ampliação do comprimento cervical de Trabalho em Primeiros testes Canais mésio-vestibular do molar superior
A ampliação dos canais radiculares utilizando diferentes tipos de métodos e instrumentos é o foco de inúmeras pesquisas na área de Deontologia. O objetivo deste estudo foi determinar a influência de quatro sistemas disti...
The importance of microsonics concept for optimization of endodontic retreatment outcome: a case report
Introduction: During the past few decades, the endodontic treatment has benefited from the development of new techniques and devices, which have improved the outcome and predictability. Important attributes such as...
Assessment of plasma and salivary antioxidant status in patients with recurrent aphthous stomatitis.
Objective: The purpose of this study was to evaluate the antioxidant levels in plasma and saliva of patients with recurrent aphthous stomatitis (RAS) and healthy controls. Material and methods: Forty patients with RAS an...
The performance of ProTaper system during the endodontic retreatment
The development of new rotary instruments for endodontic treatment demands new studies to evaluate their efficacy on the retreatment of root canals.The scope of this study was to analyze the partial or total removal of t...