A STUDY OF COMPARATIVE ANALYSIS AMONG DNA CONTENT IN DRUPE, POME, HESPIRIDIUM, PEPO AND COMPLEX FRUITS
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2018, Vol 5, Issue 1
Abstract
Genomic DNA extraction is an important aspect of plant molecular biological research. The objective of the study was to recommend the cheap and efficient genomic DNA extraction method for some economically important fruit species of Sri Lanka. The modified plant genomic DNA extraction methods explained bydrupe, pome, hespiridium, pepo and complex fruits extraction kit (Qiagen) method were applied with nine different fruit species such as Carica papaya (Papaya), Musa aouminata (Banana), Scolytus unipinosus (Grape), Psidium guajava (Guava), Malus angustifolia (Apple), Citrus aurantiam (Orange), Manilk arazapota (Whitelead), Pyrus commonis (Pear), Anaras conassus (Pineapple).Based on the quantity of the extracted genomic DNA tested by measuring the absorbance at 260 nm using Nanodrop® ND-1000 spectrophotometer, quality determined by the ratio of A260 / A280 and the amplifiable quality of DNA determined by the horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V, the method explained by Cheng et al and the Genomic DNA extraction kit yielded good quality DNA with satisfactory concentration for all the fruit species tested. Therefore the modified method of Cheng et al, 1987 could be recommended for the efficient and cost effective DNA extraction from fruit species instead of the commercially available expensive and chemically hazardous DNA easy plant kit method.
Authors and Affiliations
Naznin Pervin
Keywords
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