A study on simultaneous measurement method for both platelets and fragmented red cells using flow-cytometric immunoplatelet count
Journal Title: Journal of Analytical Bio-Science - Year 2013, Vol 36, Issue 5
Abstract
Simultaneous measurement of both platelets and fragmented red cells (FRCs) using the flow-cytometric immunoplatelet (CD41/CD61) count was attempted. FRCs were prepared artificially by heating. The ICSH/ISLH immunoplatelet count obtained by flow cytometry was extended to count FRCs, and the finding was compared to that obtained with the visual microscope-dependent method using the Miller ocular disk. A new region was established to enumerate the FRCs on the scattergram for immunoplatelet counting by flow cytometry. A favorable correlation and regression were obtained between FRC% by flow-cytometric immunoplatelet counting and that by counting on microscopic observation. Simultaneous measurement of both platelets and FRCs was possible up to a 3.8% FRC concentration.
Authors and Affiliations
Hiroshi Kondo
Keywords
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