A STUDY ON THE DETECTION OF BIOFILM FORMATION BY MULTIDRUG RESISTANT UROPATHOGENIC ISOLATES IN A TERTIARY CARE HOSPITAL
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 80
Abstract
BACKGROUND Urinary Tract Infections (UTI) are the most common and important nosocomial infections, especially among diabetic patients. Emergence of multi-drug resistance and biofilm formation by these pathogens lead to chronic and recurrent infections. Our study aims at detection of multi-drug resistant uropathogens and biofilm formation by them. Aims and Objective- Identification and isolation of significant multi-drug resistant uropathogens in diabetic patients. Detection of biofilm formation by these multi-drug resistant uropathogens by TCP, TM and CRA methods and comparison of these three methods for their efficacy. Settings and Design- This is a hospital-based prospective cross-sectional study carried out among the Diabetic patients suffering from UTI in a tertiary care hospital for a period of six months. MATERIALS AND METHODS This study involved 127 Diabetic patients of both Type I and Type II, suffering from UTI. The urine samples collected were processed by standard microbiological techniques and the antibiotic susceptibility pattern of significant uropathogens were assessed. Uropathogens resistant to any three or more of the commonly used anti-microbial agents were considered multi-drug resistant and were assessed for biofilm formation by the following three methods- Tissue Culture Plate (TCP) method, Tube Method (TM) and Congo Red Agar (CRA) method using Pseudomonas aeruginosa ATCC 27853 strain and Staphylococcus epidermidis ATCC 12228 strains as controls. The results were analysed using the standard statistical methods. RESULTS In our study, the prevalence of UTI was higher in females (56.69%) and in Type II Diabetic patients (91.34%). Among the isolates, Escherichia coli was the commonest uropathogen. About 52% of the isolates from urine samples were multi-drug resistant showing resistance to Penicillin, Cephalosporins and Sulphonamides. TCP method is found to be the standard method for the detection of biofilm formation. Biofilm formation and multi-drug resistance are found to be more in diabetic patients with poor glycaemic control. CONCLUSION Hence, this study reinforces the need for good glycaemic control in diabetic patients to prevent multi-drug resistance and biofilm formation by uropathogens.
Authors and Affiliations
Samidurai Nalayini, Moongilpatti Ramasami Vasanthapriyan, Nagarajan Anuradha, Daev Aravindh Moorthi
Keywords
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- EP ID EP268159
- DOI 10.14260/jemds/2017/1223
- Views 79
- Downloads 0
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