COMPARATIVE STUDY OF TOTAL LEUCOCYTE COUNT AND GRANULOCYTE, AGRANULOCYTE COUNT IN DIFFERENT AGE GROUPS DURING MENSTRUAL CYCLE
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2019, Vol 8, Issue 20
Abstract
BACKGROUND Menstrual cycle is the cyclical phenomenon characterized by periodic vaginal bleeding. It influences all the system of the body. Menstruation occurs cyclically at intervals of 21-35 days.Uterine cycle includes menstruation, proliferative and secretory phases. It is due to invisible interplay of different levels of hormones mainly through hypothalamo-pituitary-ovarian-axis. The physiological levels of oestrogen and progesterone influence the leucocyte count. Thus, change in the count might occur when the hormonal balance changes during menstrual cycle and most commonly the organs in the body remain unaffected by this hormonal imbalance. We wanted to study the age-related variations in total leucocyte, granulocyte and agranulocyte count during different phases of menstrual cycle in 1st professional MBBS students at, Jawaharlal Nehru Institute of Medical sciences (JNIMS), Imphal, Manipur. METHODS It is a longitudinal study. 50 healthy female medical students in the age group of 18-25 yrs. with regular cycles of 28±2 days duration participated in the study. Students with irregular cycles, gynaecological disorders like endometriosis, fibroid, irregular bleeding, history of prolonged drug intake and history of fever were excluded from the study. Study protocol was explained to the students and informed consent was obtained from each of the students. Institutional ethical committee clearance was obtained. First sample was collected within 48 hours of onset of menstruation, second sample during 8th-10th day of menstrual cycle. Third sample was taken during 22nd-24th day of menstrual cycle. All the subjects were followed up during a single cycle. Samples were taken in the morning between 9 am-10 am to avoid diurnal variation. Total leucocyte count was determined with Improved Neubauer's chamber using Turk's fluid. Granulocyte- and agranulocyte-count was determined using Leishman's stain under compound microscope. Total leukocyte count and granulocyte and agranulocyte cells were examined during a single cycle. Data collected was statistically analysed. RESULTS There was increase in total leukocyte count in the 23-25 years age group in different phases of the menstrual cycle as compared to other age groups but was not statistically significant and increased granulocyte count was seen in both the aged groups (17-19), (23-25) years in all phases of menstrual cycle was statistically significant. Agranulocyte count was increased in 23-25 years age group in all phases but was not statistically significant. CONCLUSIONS This study was an attempt to determine the normal variations in the total leucocyte count and granulocyte-, agranulocyte-count in different aged groups during the different phases of the menstrual cycle which may help in understanding various autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus, autoimmune thyroid disease, multiple sclerosis etc.) occurring in reproductive age groups.
Authors and Affiliations
Sumpi Rosemary Anal, Kanmi Ningshen, Laishram Leimahanbi Chanu
Keywords
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- EP ID EP617741
- DOI 10.14260/jemds/2019/360
- Views 88
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