A VALIDATED REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DOXOFYLINE IN PURE AND PHARMACEUTICAL FORMULATIONS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 2
Abstract
A novel reverse phase high performance liquid chromatographic method (RP-HPLC) was developed and validated for the determination of doxofylline (DXF) in pharmaceutical formulations. Agilent 1100 series HPLC instrument equipped with variable wavelength programmable UV-Visible detector and a chromosil C18 (250mm x 4.6mm, 5μm) column with an auto injector was used for the present investigation. A volume of 20µL of working standard of concentration 25µg/mL was injected into the chromatographic system, the component was separated by using the mobile phase of the mixture of potassium dihydrogen phosphate buffer of pH 5.5 and acetonitrile in the ratio 75:25(v/v) at a flow rate of 1.0 mL/min and the detection of the components was carried out at a wavelength of 275nm. Chemstation software was used for the data acquisition through out the analysis. The system suitable parameters such as number of theoretical plates, tailing factor and resolution were found to be satisfactory. The retention time of the component was found to be at 4.814 minutes. The proposed method was validated in terms of precision, accuracy, linearity, limit of detection, limit of quantification, robustness and ruggedness. The developed method was found to be rapid, simple and sensitive hence it could be used as an alternative method in assay of the doxofylline in any pharmaceutical industries.
Authors and Affiliations
Venkateswararao Lanka , Ramu Golkonda , Rambabu Chintala
Keywords
Related Articles
- EP ID EP93163
- DOI -
- Views 112
- Downloads 0
MICROWAVE ASSISTED GREEN SYNTHESIS OF SILVER NANOPARTICLES USING BOSWELLIA SERRATA FLOWER EXTRACT AND EVALUATION OF THEIR ANTIMICROBIAL ACTIVITY
In the present investigation, we describe a cost effective green synthesis of silver nanoparticles from the extract of Boswellia serrata flower as reducing agent. The formed silver nanoparticles were characterized using...
HEPATOTOXICITY V/S HEPATOPROTECTIVE AGENTS – A PHARMACOLOGICAL REVIEW
Liver is the principle organ for maintaining the body’s internal environment. Its major influence on the flow of nutrients, & control the metabolism of carbohydrate, proteins and fats. It is also the major reticulo-...
ANTICANCER ACTIVITY OF HYDROALCOHOLIC EXTRACTS FROM PARIS POLYPHYLLA RHIZOMES AGAINST HUMAN A549 LUNG CANCER CELL LINES USING MTT ASSAY
The following study is designed to determine the anticancer and cytotoxic potential of hydroalcoholic extract from rhizomes of Paris polyphylla to produce any cytotoxic effect on human A549 lung cancer cell lines. The hy...
TREATMENTS FOR ALZHEIMER’S DISEASE: AN OVERVIEW
Several diagnostic procedures have been carried out for implementing a successful treatment procedure for Alzheimer’s disease. Most of the researches went in vain and many other scientists continued their researches for...
DABIGATRAN ETEXILATE: NEW DIRECT THROMBIN INHIBITORS ANTICOAGULANTS
Thrombin plays a key role in thrombotic events, and therefore thrombin inhibition represents a therapeutic target for numerous thromboembolic diseases. Thrombin is responsible for the conversion of soluble fibrinogen to...