A VALIDATED REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DOXOFYLINE IN PURE AND PHARMACEUTICAL FORMULATIONS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 2
Abstract
A novel reverse phase high performance liquid chromatographic method (RP-HPLC) was developed and validated for the determination of doxofylline (DXF) in pharmaceutical formulations. Agilent 1100 series HPLC instrument equipped with variable wavelength programmable UV-Visible detector and a chromosil C18 (250mm x 4.6mm, 5μm) column with an auto injector was used for the present investigation. A volume of 20µL of working standard of concentration 25µg/mL was injected into the chromatographic system, the component was separated by using the mobile phase of the mixture of potassium dihydrogen phosphate buffer of pH 5.5 and acetonitrile in the ratio 75:25(v/v) at a flow rate of 1.0 mL/min and the detection of the components was carried out at a wavelength of 275nm. Chemstation software was used for the data acquisition through out the analysis. The system suitable parameters such as number of theoretical plates, tailing factor and resolution were found to be satisfactory. The retention time of the component was found to be at 4.814 minutes. The proposed method was validated in terms of precision, accuracy, linearity, limit of detection, limit of quantification, robustness and ruggedness. The developed method was found to be rapid, simple and sensitive hence it could be used as an alternative method in assay of the doxofylline in any pharmaceutical industries.
Authors and Affiliations
Venkateswararao Lanka , Ramu Golkonda , Rambabu Chintala
STABILITY EVALUATION OF TOPICAL OINTMENT COMPRISING CALCIPOTRIOL AND PREDNICARBATE
Stability studies ensuring the maintenance of product quality, safety and efficacy throughout the shelf life are considered as pre-requisite for the acceptance and approval of any pharmaceutical product. The objective of...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF PRASUGREL IN BULK AS WELL IN PHARMACEUTICAL DOSAGES FORM
This study was designed to develop and validate a simple, sensitive, precise, and specific reverse phase high-performance liquid chromatographic (HPLC) method for the determination of Prasugrel in bulk and its tablet dos...
MIRACULOUS THERAPEUTIC EFFECTS OF HERBAL DRUGS USING NOVEL DRUG DELIVERY SYSTEMS
Plants are nature’s remedies and have been used by human beings on earth since ancient times for food and medicine. Today there are global movements towards finding of herbal medicaments in plants on lab scale and after...
ROLE OF VARIOUS RISK FACTORS ASSOCIATED WITH CARDIOVASCULAR DISEASES
Coronary Artery Disease (CAD) is the leading cause of cardiovascular mortality world wide. Increasing rate of CAD mortality and projected rise in CAD mortality for 2020 in the developing world necessitates immediate pre...
SYNTHESIS THROUGH MICROWAVE IRRADIATION, CHARACTERIZATION AND EVALUATION OF ANTIMICROBIAL ACTIVITY OF 2-PHENYL-1, 3-BENZOXAZOLE DERIVATIVES
A series of 2-phenyl-1,3-benzoxazoles were synthesized by the reaction of 2-aminophenol and acyl chlorides using microwave irradiation. Purity of compounds was determined by TLC. All the synthesized compounds were cha...