A VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF DILTIAZEM IN RAW MATERIAL AND PHARMACEUTICAL DOSAGE FORM
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2018, Vol 10, Issue 6
Abstract
The objective of this work is to develop and validate a reverse phase high performance liquid chromatography (RP-HPLC) method for the quantitative analysis of Diltiazem in bulk and pharmaceutical dosage form. Chromatographic analyses were performed on RP C-18 column with a mobile phase consisting of 0.01M ammonium acetate in water, methanol and acetonitrile in the ratio 700:240:60 at a flow rate of 1 mL/min. The Diltiazem was detected and quantitated using a photodiode array detector at a wavelength of 295 nm with a retention time of 11.57 min. The detector response was linear in the concentration of 20-60µg/ml, the respective linear regression equation being Y=3000181x+356238.2. The limit of detection and limit of quantification were 0.5µg/ml and 0.15µg/ml respectively. The assay of Diltiazem in bulk was found to be 99.85%. From the recovery studies it was found that about 101% on average of Diltiazem was recovered which indicates high accuracy of the method. The method was validated by determining its accuracy, precision and system suitability.The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of Diltiazem in bulk and pharmaceutical dosage form.
Authors and Affiliations
B M S Kumar, B. Rajkamal
Keywords
Related Articles
- EP ID EP622236
- DOI 10.25004/IJPSDR.2018.100609
- Views 70
- Downloads 0
DETERMINATION OF THE RUTIN, QUERCETIN AND KAEMPFEROL CONTENTS IN A GHANAIAN POLYHERBAL FORMULATION (EAF-2011) AND ITS RAW MATERIALS USING A SIMULTANEOUS RP-HPLC METHOD
Quantitative chemical fingerprinting of herbal medicines provides an avenue for addressing the challenge with their quality. In this report, chemical markers that could be labelled as active markers in the polyherbal pro...
OPTIMIZATION OF CILNIDIPINE NANOSUSPENSION USING A CENTER COMPOSITE DESIGN
The purpose of this investigation was to increase the solubility and dissolution rate of Cilnidipine by the preparation of nanosuspensions with solvent antisolvent method at the laboratory scale. Drug solution of Cilnidi...
THERAPEUTIC L-ASPARAGINASE ACTIVITY OF BACTERIA ISOLATED FROM MARINE SEDIMENTS
L-Asparaginase, a therapeutic enzyme used in lymphoblastic leukemia and lymphosarcoma chemotherapy which is derived mostly from the bacterial sources Escherichia coli and Erwinia sp. The long term administration of the d...
Photooxidized Vespa orientalis venom Improves Memory and Learning Activities in Rats
The work was aimed to study the effect of photooxidized Vespa orientalis venom on memory and learning activity on rats in presence of scopolamine and ondansetron using T maze, Elevated plus maze and passive avoidance. UV...
EXTENDED RELEASE MICRO-PELLETS OF CHIRAL MOLECULE OF METOPROLOL SUCCINATE BY FLUID BED TECHNOLOGY
The objective is to prepare extended release micro-pellets of the s-Metoprolol Succinate which is chirally pure molecules. Commercially Metoprolol Succinate is available the racemic mixture of the s and r isomers. Out of...