Activation and IL-1b secretion of human peripheral phagocytes infected with Actinomadura madurae, Nocardia asteroides and Candida albicans
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2016, Vol 9, Issue 10
Abstract
Objective: To evaluate the ability of Actinomadura madurae (A. madurae) and Nocardia asteroides (N. asteroides), using Candida albicans (C. albicans) as prototypic control, to elicit the activation and IL-1b secretion of blood phagocytic cells from healthy donors. Methods: Microscopic evaluation of phagocytosis/activation, cell viability and spectrophotometric quantitation of endocytosis/activation, were assessed by using formazan blue test in human blood phagocytes infected with C. albicans, A. madurae or N. asteroides treated with either normal human serum (NHS) or with decomplemented NHS. Interlukin-1b from culture supernatants of infected polymorphonuclear was tested by ELISA kit assay. Results: Microscopic assay showed that phagocytosis and activation of adherent mononuclear phagocytes were greater with C. albicans followed by A. madurae and then by N. asteroides. Spectrophotometric assay in polymorphonuclear phagocytes infected with NHS-treated pathogens indicated that activation was similarly higher by C. albicans and A. madurae and lower by N. asteroides. Kinetic assays in infected polymorphonuclear cells showed that viability was decreased by C. albicans and N. asteroides or unaffected with A. madurae. Levels of IL-1b at 8 h of incubation were higher with C. albicans followed by A. madurae whereas lower levels were found with N. asteroides. Conclusions: The extent of cell-viability and activation as well IL-1b secretion may be related with the virulence of C. albicans and N. asteroides and other parameters remain to be explored for assessing the virulence of A. madurae.
Authors and Affiliations
Alejandro Palma-Ramos, Teresita Sainz-Espuñes
Keywords
Related Articles
- EP ID EP282577
- DOI 10.1016/j.apjtm.2016.07.026
- Views 97
- Downloads 0
Traditional usage, phytochemistry and pharmacology of Croton sylvaticus Hochst. ex C. Krauss
Croton sylvaticus (C. sylvaticus) is widely used as herbal medicine by the indigenous people of tropical Africa. The potential of C. sylvaticus as herbal medicine, the phytochemistry and pharmacological properties of its...
Antibiofilm activity of a-mangostin extracted from Garcinia mangostana L. against Staphylococcus aureus
Objective: To isolate a-mangostin (AMG) from the peels of mangosteen (Garcinia mangostana L.), grown in Vietnam, and to investigate antibiofilm activity of this compound against three Staphylococcus aureus (S. aureus) st...
Effect of tetramethylpyrazine combined with cisplatin on VEGF, KLF4 and ADAMTS1 in Lewis lung cancer mice
Objective: To further explore the function of combine use of tetramethylpyrazine (TMP) and cisplatin (DDP) in lung carcinoma. Methods: We used the combination drug to treat Lewis lung cancer mice, investigated the expres...
The role of heme-oxygenase-1 in pathogenesis of cerebral malaria in the co-culture model of human brain microvascular endothelial cell and ITG Plasmodium falciparum-infected red blood cells
Objective: To investigate the role of human host heme-oxygenase-1 (HO-1) in pathogenesis of cerebral malaria in the in vitro model. Methods: The effect of human host HO-1 [human brain microvascular endothelial cell (HBME...
A single administration of fish oil inhibits the acute inflammatory response in rats
Objective: To investigate the antiinflammatory effects of a single administration of fish oil (FO) on the acute inflammatory response. Methods: The paw edema and pleurisy models were used to evaluate the effects of FO di...