Active Phytochemical Detecting, Antioxidant, Cytotoxic, Apoptotic Activities of Ethyl Acetate and Methanol Extracts of Galium aparine L.
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 15, Issue 6
Abstract
The aim of this study was to detect active phytochemicals in ethyl acetate (EAE) and methanol (ME) extracts of Galium aparine L. and determine in vitro antioxidant, cytotoxic and apoptotic activities of these extracts. The phytochemicals present in the plant were determined using standard methods and HPLC. EAE and ME of Galium aparine arial parts were tested for antioxidant (by using DPPH radical scavenging, H2O2 scavenging and metal chelating assays), cytotoxic (by using MTT assay on MCF-7 breast cancer and Caco-2 colon cancer cells), and apoptotic activity (DNA diffusion assay on MCF-7 breast cancer and Caco-2 colon cancer cells). Human peripheral blood lymphocytes were used as control cells. As a result of phytochemical screening of EAE and ME of Galium aparine arial parts, phenols, tannins, alkaloids, anthraquinones and saponins were detected. Furthermore, rutin, a flavonoid phytochemical was found in both of the extracts by HPLC analysis. EAE and ME have cytotoxic and apoptotic activity on human MCF-7 breast cancer and Caco-2 colon cancer cells. These activities may depend on polarity of solvents used in the extraction and on concentration of the extracts. EAE and ME showed strong DPPH• radical scavenging, very low H2O2 scavenging and metal chelation activity. Besides, EAE showed higher cytotoxic and apoptotic effect on human peripheral lymphocytes compared to ME. The G. aparine EAE and ME led cytotoxicity and apoptosis in human MCF-7 breast cancer and Caco-2 colon cancer cells.
Authors and Affiliations
Özlem Sultan Aslantürk, Tülay Aşkin Çelik, Burçin Karabey, Fatih Karabey
Keywords
Related Articles
- EP ID EP312932
- DOI 10.9734/BJPR/2017/32762
- Views 134
- Downloads 0
Antifungal Activity of Ziziphus mucronata and Erythrina abyssinica Bark Crude Extracts on Cryptococcus neofomans and Candida albicans Species
Aims: The study aims at extraction, characterization and identification of bioactive compounds with antifungal properties from the plant species E. abyssinica and Z. mucronata against Cyptococcal neoformans and Candida a...
Dose Response of Viola odorata on Meiotic and Mitotic Chromosomes of Vicia faba
Aims: Viola odorata is a medicinally important plant. It contains bioactive principle viz violin and probably methyl salicylic ester. The present study was designed to analyze the effect of different doses of this medici...
TNF-α Inhibitor Treatment for Crohn’s Disease: Comparative Review of Post Therapy Malignancy between Infliximab and Adalimumab
The association between chronic inflammatory disease and cancer has been well established through years of research. In corollary, progressive resistance to chimeric monoclonal antibodies has been reported in literature....
Haematological and Biochemical Properties of Methanolic Extract of Citrullus lanatus Seeds
Watermelon is a commonly consumed, but this consumption is usually limited to the fruit. Phytochemical constituents of watermelon have known advantages, however the seeds of the plant are usually thrown away. This study...
Inhibition of Albumin Glycation at Different Stages by Four Anti-diabetic Plant Extracts Correlates with Polyphenols and Antioxidant Capacity in vitro
Aims: Both glycation and oxidative stress play a critical role in the incidence of diabetic complications. Plants with antioxidative and antiglycative properties may attenuate such pathological conditions. The aim of thi...