Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis

Apply

Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis

Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 14, Issue 2

Abstract

Aims: The aim of this study was to investigate the possible hypotensive and diuretic effects of ethanolic extract of Urtica circularis (Hicken) Sorarú (Urticaceae) using preclinical methods. Place and Duration of Study: Department of Pharmacology, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, from July 2015 to January 2016. Methodology: Effect on blood pressure and heart rate on anaesthetized normotensive and hypertensive rats were measured using Statham Gould P23ID pressure transducer coupled to a Grass 79D polygraph. Rats were placed in metabolic cages in order to collect urine. Urinary volume was measured and sodium and potassium concentration was estimated from each urine sample using indirect ion-selective electrode potentiometry. The vasorelaxant activity of major compound was studied using isolated aortic rings. Antioxidant activity was estimated measuring 2,2 diphenyl 2 picryl hydrazyl hydrate radical scavenging activity. Results: The intravenous administration of the extract of U. circularis (0.1–30 mg/kg) in anaesthetized normotensive and hypertensive rats caused a dose-dependent reduction in the mean arterial pressure without affecting the heart rate. The greater reduction of blood pressure induced by U. circularis was observed in hypertensive rats (30 mg/kg: Spontaneously Hypertensive Rat: -34.7±3.3 mmHg, Spague Dawley: -18.3±3.9 mmHg). Cumulative urinary excretions 24 h after treatment with the extract 100 and 300 mg/kg were 18.2±1.2 and 14.9±1.5 mL respectively, significantly higher than the control group (9.0±1.3 mL). The addition of cumulative concentrations of vicenin-2 (10-7-10-4M) generated relaxation in endothelium-intact aortic rings pre-contracted with 10–7M Phenylephrine (Emax = 66.2±3.5%). Extract showed antioxidant activity reaching 45% of DPPH scavenging activity at 1000 μg/mL, meanwhile the flavonoid reached 20% of scavenger capacity. Conclusion: U. circularis, has a diuretic, antioxidant and hypotensive effect. Vicenin-2, the major component of this extract showed vasorelaxant activity, potentially responsible for the properties of the extract.

Authors and Affiliations

A. Rodríguez Basso, C. Marrassini, S. Gorzalczany

Keywords

Hypotensive; diuretic; vasorelaxant; antioxidant; Urtica circularis; vicenin-2.

2, 2-Diphenyl-1-picrylhydrazyl Radical Effect and Phytochemical Constituents of Combretum platypetalum Welw. ex. M. A. Lawson subsp. oatesii (Rolfe) Exell Leaf

Aims: To investigate the phytochemical constituents and antioxidant properties of Combretum platypetalum leaf. Study Design: In vitro assessment of antioxidant properties and determination of the phytochemical constitue...

Evaluation of Free Radical Scavenging Activity and Toxic Heavy Metal Contents of Commercially Available Fruits of Tribulus terrestris Linn.

Aims: To evaluate the free radical scavenging activity of organic solvent extracts and the toxic heavy metal contents of commercially available fruits sample of Tribulus terrestris Linn in the UAE. Methodology: Fruit sam...

EP ID EP349700
DOI 10.9734/EJMP/2016/25661
Views 123
Downloads 0