HPLC Determination of the Eight Constitutes in Portulaca oleracea L. from Different Locations
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 5, Issue 2
Abstract
Aims: The objective of the present research was to simultaneously determine the eight constituents in Portulaca oleracea L. from different locations by a sensitive and specific high-performance liquid chromatographic (HPLC) method, including caffeic acid (CA), p-coumaric acid (p-CA), scopoletin (SCOP), ferulic acid (FA), quercetin-3-O-rhamnoside (QR), quercetin (QUER), apigenin (APIG) and bergapten (BERG) using rhein as the internal standard. Methodology: With gradient elution procedure and mobile phase A (0.1% formic acid) and B (acetonitrile) ratio linear changing, the wavelength was set to 320 nm to determinate the eight constituents. Result: The method validation presented good accuracy with recoveries in the range of 93.67-113.3% and good precision with RSD values less than 12.1%.The results indicated that there existed the significant differences of the eight constituents in P. oleracea L. from 20 different locations. Conclusion: The results can be used to reasonably assess the drug quality and exploit P. oleracea L.
Authors and Affiliations
Junjun Ai, Aijing Leng, Xiaomei Gao, Wenjie Zhang, Di Li, Liang Xu, Xixiang Ying
Keywords
Related Articles
- EP ID EP350286
- DOI 10.9734/EJMP/2015/13253
- Views 140
- Downloads 0
Antitrypanosomal Activity of Allium porrum Ethyl Acetate Bulb Extract in Trypanosoma brucei brucei Experimentally Infected Wister Albino Rats
Aims: The present study investigated the antitrypanosomal activity of crude ethyl acetate bulb extracts of A. porrum plant against T. b. brucei. Methodology: Twenty five male albino rats were divided into five groups an...
Ergot Alkaloids: A Review on Therapeutic Applications
Ergot of Rye is a plant disease caused by the fungus Claviceps purpurea which infects the grains of cereals and grasses but it is being used for ages for its medicinal properties. All the naturally obtained ergot alkaloi...
Isolation and Chemical Structural Characterization of the Mixture of Two Related Phytosterols from Ricinus communis L. (Euphorbiaceae) Leaves
Aim: Ricinus communis, a member of the family Euphorbiaceace, is widely used in South African traditional medicine for the treatment of wounds, sores and boils. The plant was selected based on a high antibacterial activi...
Inhibitory Activity of Leaves Extracts of Citrullus colocynthis Schrad. On HT29 Human Colon Cancer Cells
Aims: Citrullus colocynthis is a plant endemic in Asia, Africa and in the Mediterranean basin. It is used in folk medicine against infections, inflammations and cardiovascular and immune-related diseases. There are furth...
Acaricidal Activities of Hyptis suaveolens and Ocimum sanctum Against African Dog Tick (Rhipicephalus sanguinneus)
Aim: To determine the acaricidal activities of some solvent extracts (chloroform, methanol and n-hexane) and crude extracts of Hyptis suaveolens and Ocimum sanctum against African dog tick (Rhipicephalus sanguinneus). S...