ACUTE, SUB-ACUTE AND ANTIOXIDANT ACTIVITIES OF ARTHROPHYTUM SCOPARIUM AERIAL PARTS
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 9
Abstract
This study aims to evaluate the in-vitro and in-vivo antioxidant activity of different extracts prepared from the aerial parts of Arthrophytum scoparium. Polyphenols flavonoids and tannins contents were determined. The acute toxicity of ASE was carried out based on OECD during two weeks to ascertain the LD50. In sub-acute toxicity, a 100, 200 and 400 mg/kg/day extract was orally administrated for 21 days consecutively. The results showed that decoction extract (DEC) was the richest fractions in Polyphenols, flavonoids and tannins content (23.29 mg/GAE, 99.77 mg/QE and 113.33 mg/TAE), respectively. Arthrophytum scoparium hydro-methanolic extract (ASE) had the strongest antioxidant activity using DPPH and ABTS scavenging assays with an IC50 of 0.045 and 0.0017 mg/ml, respectively. Moreover, the β-carotene/linoleic acid bleaching assay showed that ethyl acetate extract (EAE) had the highest inhibiting activity (83.29%). Furthermore, all A. scoparium fractions exhibited a good ferric reducing power. The administrated doses did not undergo changes in general behavior, toxicity or mortality of the tested mice. The LD50 was found to be superior to 5 g/kg. Daily administration of ASE leads to an increase in GSH level and decreasing the lipid peroxidation in the liver. In-vivo, ASE showed a strong scavenging effect on DPPH and reducing power. In conclusion, A. scoparium extracts exhibited considerable antioxidant effects in-vitro and in-vivo. These findings support the traditional use of this plant as an anti-inflammatory in therapeutics.
Authors and Affiliations
S. M. Kaddour et al.
TO EVALUATE THE USE AND SAFETY OF BLOOD THINNING AGENTS IN TERTIARY CARE HOSPITAL
Objective: To ensure the basic principles of drug utilization and the use and safety of blood thinning agents in tertiary care hospital. Experimental design: The study included 224 patients’ and was conducted in a tertia...
A REVIEW ON GENOTOXICITY, ITS MOLECULAR MECHANISMS, REGULATORY TESTING IN DRUG DEVELOPMENT PROCESS
Genotoxicity has fully-grown into a serious question for the cause of several cancers. In this article, we discuss the basics of genotoxicity, the chemicals which cause these genetic damages and also their mechanism of a...
ACUTE, SUB-ACUTE AND ANTIOXIDANT ACTIVITIES OF ARTHROPHYTUM SCOPARIUM AERIAL PARTS
This study aims to evaluate the in-vitro and in-vivo antioxidant activity of different extracts prepared from the aerial parts of Arthrophytum scoparium. Polyphenols flavonoids and tannins contents were determined. The a...
DEVELOPMENT AND INVESTIGATION OF THERMO-SENSITIVE ORGANOGEL OF DICLOFENAC SODIUM FOR IN-SITU IMPLANTATION
The aim of the present study was to develop and evaluate thermo-sensitive in-situ implant forming injectable organogel systems of diclofenac sodium for prolonged drug effects. The formulations were prepared by separately...
DRUG UTILIZATION PATTERN IN GERIATRIC PATIENTS OF GENERAL MEDICINE IN A SECONDARY CARE HOSPITAL
The purpose of the research was to assess the prescribing pattern in geriatrics and to analyze the prescription using World Health Organization (WHO) core prescribing indicators and American Geriatric Society (AGS) Beers...