ACUTE TOXICITY OF DIFFERENT SIZES OF SILVER NANOPARTICLES INTRAPERITONALLY INJECTED IN BALB/C MICE USING TWO TOXICOLOGICAL METHODS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: This study aimed to evaluate the acute toxicity of intraperitoneally administrated silver nanoparticles (AgNPs) with different particle sizes in BALB/c mice.Methods: Citrate-capped AgNPs were prepared by citrate reduction method and isolated into small particles (average size 20 nm) and large particles (average size 50 nm). The median lethal dose (LD50) of 20 nm and 50 nm AgNPs was estimated using two toxicological methods, classical Dixon's up-and-down method and AOT425statPgm method for up-and-down procedure.Results: The LD50 was evaluated at the dosage level of 169 and 213.8 mg/kg, respectively for 20 nm AgNPs and at the dosage level of 354 and 391.5 mg/kg, respectively for 50 nm AgNPs. The results showed that LD50 obtained by the AOT425statPgm method was in accord with that of the Dixon's method and no significant differences between them (P = 0.06). The size 20 nm AgNPs were more toxic than the size 50 nm AgNPs. The behavioural responses and deviations were dose dependent, increasing by increasing the dose. The anatomical examinations showed that AgNPs were mainly accumulated in liver and spleen of dosed mice.Conclusion: The results suggested that the AOT425statPgm method was an efficient tool and a good alternative method for use in future acute toxicity studies.Â
Authors and Affiliations
Elham A. Elkhawass, Mahmoud E. Mohallal, Maha F. m. Soliman
Keywords
Related Articles
- EP ID EP579042
- DOI -
- Views 61
- Downloads 0
ANTIBIOTICS PRESCRIPTION PATTERN IN RURAL AREA OF BANGLADESH: A CROSS-SECTIONAL STUDY IN DEBIDWAR UPAZILA OF COMILLA DISTRICT
Objective: The study was aimed to evaluate the antibiotic prescription pattern by physicians in the area of study. Methods: This cross-sectional observational study was carried out with a self-designed standard question...
SILVER NANOPARTICLES SYNTHESIS FROM DIFFERENT FUNGAL SPECIES AND THEIR ANTIFUNGAL EFFECT
Objective: Evaluation of antifungal activity of silver nanoparticles synthesized using different fungal speciesMethods: Fungus like Fusarium oxysporum, Fusarium udum, Fusarium oxysporum and Stemphylium vericans were used...
SCREENING OF CHITINASE PRODUCTION AND ANTIFUNGAL ACTIVITY OF STREPTOMYCES SP. ACT7 FROM EAST COAST REGION, SOUTH INDIA
Objective: The objective of the present study was aimed to isolate actinobacteria from the marine environment and to screen the production and antifungal activity of chitinase enzyme against phytopathogenic fungi.Methods...
ANTIFUNGAL EFFECT OF HYPTIS SUAVEOLENS OIL MICROEMULSION BASED CARBOXYMETHYL MUNGBEAN GEL FOR TOPICAL DELIVERY
Objective: Conventional topical antifungal formulations limit the effectiveness of antifungal therapy. The aim of this study was to formulate effective antifungal microemulsion of H. suaveolens oil based carboxymethyl mu...
BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF SOFOSBUVIR FROM HUMAN PLASMA
Objective: This study points to build up and validate a simple methodology to quantify the most used drug sofosbuvir for the treatment of hepatitis C virus (HCV) infection, in human plasma by using atazanavir as an Inter...