ACUTE TOXICITY, PHYTOCHEMISTRY AND ANTIBACTERIAL ACTIVITY OF AQUEOUS AND ETHANOLIC LEAF EXTRACTS OF CASSIA ALATA LINN
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 6
Abstract
Cassia alata Linn is an important medicinal plant as well as an ornamental flowering plant that has diverse reported medicinal values. This study focused on the evaluation of the phytochemical components, antibacterial as well as acute toxicity studies. The leaves of the plant were collected, dried, ground and extracted using 95% ethanol and water. The extracts were used for acute toxicity study, phytochemical screening and antibacterial assay using cup-plate method. The result from this study revealed the presence of saponins, anthraquinones, cardiac glycosides, flavonoids, reducing sugars and terpenes. The concentrations of these bioactive components were slightly higher in ethanol than in aqueous leaf extract. The antibacterial effects produced by the extracts was dose dependent at the tested doses (20 mg to 160 mg) on Staphylococcus aureus, Escherichia coli and Salmonella typhi. The aqueous leaf extract showed higher activity on Escherichia coli than ethanol extract at 160 mg (p<0.05), whereas ethanol leaf extract had higher activity than aqueous leaf extract on Salmonella typhi at the same dose (p<0.05). The MIC for aqueous leaf extract ranged between 3.50 mg and 25.15 mg, while that of ethanol leaf extract was from 1.41 mg to 3.55 mg on the organisms tested. This study showed that ethanol and aqueous leaf extracts contain some secondary bioactive metabolites that may be responsible for the observed antibacterial activity and thus supports the traditional use of the plant in the management of typhoid fever and other infections caused by susceptible organisms.
Authors and Affiliations
SY Timothy , FW Lamu , AS Rhoda , RG Adati , ID Maspalma , M Askira
Keywords
Related Articles
- EP ID EP135231
- DOI -
- Views 107
- Downloads 0
EVALUATION OF ANTI-OXIDANT, CYTO-TOXIC AND ANTI-MICROBIAL PROPERTIES OF DRYNARIA QUERCIFOLIA
Drynaria quercifolia is a plant having wide spread ethno pharmacological uses. The aim of this study was to evaluate the anti-oxidant, anti-microbial and cyto-toxic activities of the whole plant Drynaria quercifolia. The...
EVALUATION OF PHYTOCHEMICAL CONSTITUENT IN CONVENTIONAL AND NON CONVENTIONAL SPECIES OF CURCUMA
Plants and plant based medicaments are the basis of many of the modern pharmaceutical we use today for our various aliment. Plant show medicinal properties as it contain phytochemical constituent. Phytochemical constitue...
FORMULATION AND EVALUATION OF SUSTAINED RELEASE MATRIX TABLETS OF TRAMADOL HYDROCHLORIDE AND STUDY IT’S RELEASE (DISSOLUTION) KINETICS
The objective of this work was to develop sustained release tablets of highly water soluble Tramadol HCl using polymers (HPMC K100M, HPMC K15M, Sodium Carboxy Methyl Cellulose, Polyethyleneoxide-N-80) as cost effective,...
FERMENTATION, MEDIA OPTIMIZATION STUDIES FOR COENZYME Q10 PRODUCTION BY Saccharomyces cerevisiae
To establish the fermentation process for CoQ10 production by using Saccharomyces cerevisiae with subsequent bioprocess media optimization studies. Coenzyme Q10 (CoQ10) is a vitamin-like nutrient that plays a vital role...
ORGANOLEPTIC AND WORMICIDAL EVALUATION OF VARIOUS CRUDE EXTRACTS OF POMEGRANATE (PUNICA GRANATUM) PEEL
In the present study, organoleptic evaluation of Pomegranate (Punica granatum L) peel powder was done and found pink in color having no smell and slightly bitter taste. Moreover, aqueous and methanolic extracts of Pomegr...