ADAPTATION OF THE PROCEDURE FOR POLYPHENILENE MEMBRANE DEPOSITION ON THE DISK PLATINUM TRANSDUCERS
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2017, Vol 14, Issue 1
Abstract
The biosensor selectivity is an important factor when working with real biological fluids and other complex samples; therefore, study on the biosensor selectivity towards possible interfering compounds and its improvement is a mandatory step in the development of biosensors. In case of amperometric biosensors, the main interfering compounds are electroactive substances able to oxidize on the working electrode and generate electric current. To eliminate the impact of these compounds, the deposition of semipermeable membranes onto transducer surface may be used, the procedure being methodologically simple and having no significant effect on the biosensor performance. The aim of this work was to optimize the conditions of deposition of polyphenylenediamine (PPD) based semipermeable membrane onto amperometric transducers. Methods: Amperometric method of analysis was used. Platinum disc electrodes served as amperometric transducers, they were connected to potentiostat PalmSens (Netherlands) via three-electrode measurement circuit. PPD membranes were deposited onto transducers by phenylenediamine electropolymerisation at a constant or variable potential. Results: The transducers sensitivity to a number of electroactive substances was determined before and after deposition of the additional semipermeable membrane by different methods. The efficiency of PPD membrane deposited at different time of electropolymerisation and various concentrations of monomer m-phenylene diamine was studied. The transducers were shown to remain insensitive to the electroactive substances over long-term use and during one-week storage. Conclusion: The PPD membrane deposited under optimal conditions allows virtual elimination of transducers sensitivity to interfering substances (ascorbic acid, cysteine, uric acid and dopamine). Therefore, the transducers modified with PPD membranes can be effectively used to work with real biological samples such as blood serum assays.
Authors and Affiliations
I. S. Kucherenko, O. V. Soldatkina, D. Yu. Kucherenko, O. O. Soldatkin, S. V. Dzyadevych
Keywords
Related Articles
- EP ID EP398556
- DOI 10.18524/1815-7459.2017.1.96439
- Views 74
- Downloads 0
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