ADVANCEMENT OF THE METHOD OF NON-COMBINED DEFINITION OF NON-MIOCYIN IN CHARACTERISTICS THE METHOD OF IMMUNOFERMAL ANALYSIS
Journal Title: ВЕТЕРИНАРІЯ, ТЕХНОЛОГІЇ ТВАРИННИЦТВА ТА ПРИРОДОКОРИСТУВАННЯ - Year 2018, Vol 2, Issue
Abstract
Improving the methods for detecting antibiotics, in particular from the class of aminoglycosides, is an actual problem of modern laboratory work for the purpose of carrying out state control over their content in honey The determination of residual amounts of neomycin was carried out by ELISA on the Tecan Sunrise immunoassay analyzer (Sunrise, Austria) using the test systems for competitive immunoassay for EuroProxima Neomycin ELISA (Cat. No .: 5111NEO) (The Netherlands). It is a test kit for ELISA, complete with the necessary reagents, which are serially controlled by the ISO-9000 quality system and are intended to detect small concentrations of neomycin in honey. Our conditions for sample preparation and assessment of the suitability of the methodology were carried out in accordance with the criteria set out in the Decision of the European Commission 2002/657 / E of 12 August 2002 on the implementation of analytical methods and the interpretation of their results, and in accordance with the recommendations of the European Union Reference Laboratories in the field of control of residual quantities ( CRLs) 20/1/2010 on the assessment of the suitability of screening methods for the determination of residual quantities of veterinary drugs. For performing such actions, the following concepts are analyzed: correctness (accuracy, reliability) – the correspondence between the results of the test and generally accepted reference values; specificity - the ability of the method to distinguish analyte, which is determined from other substances; cut-off limit is the limit of the response or signal obtained when the method is used, indicating the presence of an analyte sample at the concentration level or above; robustness - the ability of the analytical technique not to be influenced by small changes controlled by the analyst in the conditions of the implementation of the methodology. For validation of this screening technique, 20 samples of the matrix (control pure honey) and 20 enriched specimens were examined with a standard neomycin solution at 30.0 μg / kg. Preparation of the samples was performed according to the developed method, and the study in accordance with the instructions to the test system provided by the manufacturer. The analysis was carried out on different days, taking into account possible changes in the operating regime, which have a direct impact on the performance of these studies. Statistical processing of the results was performed by Excel software. Validation characteristics for determining the residue of neomycin in honey samples are established, such as: the detection capability (by this screening method is 30.0 μg / kg), the cut-off level is 25.7 μg / kg. The lowest content of neomycin, which can be detected by ELISA using the test system for competitive immunoassay, is EuroProxima Neomycin ELISA (Cat. No .: 5111NEO) of 15.63 μg / kg.
Authors and Affiliations
K. S. Myagka, S. A. Tkachuk
Keywords
Related Articles
- EP ID EP455730
- DOI 10.31890/vttp.2018.02.26
- Views 135
- Downloads 0
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