AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT FUNGI ENDOFIT DARI AKAR MANGROVE (Rhizophora apiculata Blume) SECARA KLT BIOAUTOGRAFI
Journal Title: As-Syifaa Jurnal Farmasi - Year 2017, Vol 9, Issue 1
Abstract
Endophytic fungi have the ability to produce bioactive compounds one of which is antibacterial. A search of bioactive compounds in this research are the first was isolated endophytic fungi on the roots of mangrove then be purified and macroscopic order to obtain pure 6 isolates endophytic fungi. Proceed with the screening test to test bacteria and endophytic fungal isolates obtained first by IJM 2 code which provides activity against 5 test bacteria with the formation of the largest inhibition zone diameter. Then proceed with the fermentation of isolates IJM 2 for 21 days at room temperature using Potato Dextrose Broth medium after the separation to obtain a supernatant and mycelia. Then proceed with the solvent extraction using ethyl acetate and the extract obtained extract supernatant and mycelia. The extract obtained is then followed by TLC-Bioautografi testing using the eluent n-butanol: acetic acid: water (8: 1: 2). Results chromatogram profile with KLT-Bioutografi method showed antibacterial activity against bacteria Shigella dysentriae, Pseudomonas aeruginosa, Salmonella thypii, Vibrio cholerae, Stahpylococcus aureus.
Authors and Affiliations
Fitriana Fitriana, Eka Nursithya
Keywords
Related Articles
- EP ID EP540172
- DOI -
- Views 57
- Downloads 0
AKTIVITAS ANTIOKSIDAN FRAKSI RIMPANG KENCUR (Kaempferia rhizoma) DENGAN MENGGUNAKAN METODE PEREDAMAN 1,1 Diphenyl-2-picrylhydrazil (DPPH)
Kaempferia is one of the most widely used plants in society is a traditional at the medicine. Empirically Kaempferia rhizome has a special quality as a wound healer, good for digestion, antibacterial, and as an antioksid...
FORMULASI SIRUP EKSTRAK DAUN SENDOK (Plantago mayor L.) SEBAGAI EKSPEKTORAN DENGAN PARAMETER UJI MUKOLITIK
Traditional medicines that derive from the plants processed in the form of traditional herb have provided a lot of contribution to the maintenance the health of community. Greater plantain (Plantago mayor L.) is one of n...
ANALISIS KADAR TIMBAL (Pb), SENG (Zn) DAN TEMBAGA (Cu) PADA IKAN BANDENG (Chanos chanos Forsk.) YANG BERASAL DARI LABBAKKANG KAB. PANGKEP SECARA SPEKTROFOTOMETRI SERAPAN ATOM (SSA)
The research was done to analysis of amount lead (Pb), zinc (Zn) and copper (Cu) at milkfish (Chanos chanos Forsk.) from Labbakkang Pangkep by Atomic Absorbtion Spectrophotometry (AAS). Before to be analised, first to do...
EFEK ANTIINFLAMASI EKSTRAK ETANOL DAUN KAMBOJA (Plumeria rubra L.) PADA TIKUS PUTIH (Rattus norvegicus)
Inflammation is a body protective response against tissue injury which can be characterized by the formation of edema. Frangipani Leaves contain flavanoids, saponins and alkaloids. The flavanoid compound is considered to...
EKSTRAK DAUN JAMBU BIJI (Psidium guajava L.) SEBAGAI INHIBITOR PERTUMBUHAN BAKTERI ZOONOSIS Proteus mirabilis YANG DIISOLASI DARI DAGING AYAM BROILER
This research exploring the potential of antibacterial compounds from herbs to be important to do considering the number of cases of bacterial resistance. Proteus itself is one of the bacteria zoonosis or bacteria that...