ALLEVIATING EFFECTS OF ASCORBIC ACID ON LEAD TOXICITY IN GOJI (Lycium barbarum L.) IN VITRO
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2018, Vol 45, Issue 340
Abstract
Lead (Pb) is the most common heavy metal pollutant in the environment. The objective of the presented study was to investigate the ameliorative effect of exogenous 1 mM ASA on key growth and biochemical parameters in Lycium barbarum seedlings under heavy metal (Pb(NO3)2) stress in vitro. Nodal cutting with an axillaries bud were used as an explants. The results showed that lead accumulation in goji explants had negative influence on morphological parameters of plant growth, such as shoot and root length. Lead caused a significant reduction in chlorophylls and carotenoid content, increased lipid peroxidation and induced significant accumulation of proline in goji leaves. Addition to MS medium 1 mM ASA greatly alleviated Pb-induced growth inhibition and Pb-induced MDA and proline accumulation. Presence of ASA in the MS medium under heavy metal stress increased plant fresh and dry mass with no significant effect on plant water content.
Authors and Affiliations
Marcelina KRUPA-MAŁKIEWICZ, Arleta KRUCZEK, Justyna PELC2, Beata SMOLIK, Ireneusz OCHMIAN
Keywords
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