THE EFFECT OF MANNITOL AND SORBITOL ON SOYBEAN IN VITRO DEVELOPMENT
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2018, Vol 46, Issue 341
Abstract
The aim of this study was to examine an effect of osmotic stress, induced by mannitol (Mn) and sorbitol (So) in concentrations (0–200 mM) on seed germination and development of common soybean (Glycine max L.) seedlings in in vitro conditions. The analysis of the effectiveness of the porcession was made by assessing the length of the stems and roots as well as the fresh and dry mass of 3-week-old seedlings. The biometric index values differed depending on the type of substance used. While Mn at the lowest concentration (50 mM) did not affect the biometric parameters studied, So (50 mM) stimulated the growth of seedlings, root elongation and the number of leaves and fresh weight of plants. The unfavorable effect on biomass was noticed at higher concentrations of both substances. Mn (> 50 mM – <150 mM) negatively influenced the fresh and dry content of the seedlings. In turn, Mn (150 and 200 mM) also caused growth inhibition, decreased number and length of leaves. So (200 mM) significantly inhibited the growth of seedlings, reducing the dry matter.
Authors and Affiliations
Danuta KULPA, Andrzej GAWLIK, Renata MATUSZAK-SLAMANI, Małgorzata WŁODARCZYK, Romualda BEJGER, Mariola SIENKIEWICZ, Dorota GOŁĘBIOWSKA, Anna SEMENIUK
Keywords
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