AN ISOLATION, IDENTIFICATION AND DIVERSITY OF ENDOPHYTIC FUNGI FROM CATHARANTHUS ROSEUS AND SCREENING FOR THEIR L-ASPARAGINASE ACTIVITY
Journal Title: International Journal of Environment, Ecology, Family and Urban Studies (IJEEFUS) - Year 2018, Vol 8, Issue 6
Abstract
Catharanthusroseus is one of an important medicinal plant the alkaloids produced by this plant has been used in many medical applications. Many researchers have also isolated the endophytes found in this plant and also proved that some of the endophytes has the ability to produce the metabolites which the host plant produces. Hence in our present research we have made an attempt to isolate, identify and study the diversity of endophytic fungi in C.roseus and we also tried to screen the L-asparaginase producing endophytes in the same. A total of 13 different fungal isolates, 5 different mycelia sterilia and one Actinomycetes were isolated from C.roseus collected from six different locations. Leaf tissue showed greater diversity of endophytes followed by root and stem. This study suggests that endophytes are both host and tissue specific. Preliminary screening of L-asparaginase activity was done for all 58 isolates isolated from C. roseusof which 19 isolates were found to be potential of producing L-asparaginase. All these isolates were subjected for further analysis. The activity of L-asparaginase was analysed by Nesselerization method. Nigrospora sp. followed byLasiodiploidiasp. was found to be potential of producing L-asparaginase. The optimum pH and temperature required for L-asparaginase activity produced by most of the isolates was found to be pH-6 at 30°C. Hence, to the best of our knowledge this is the first report, which revealed the presence of Lasiodiploidia sp. and Madurella sp. as an endophytes in Catharanthusroseus and first report of isolation of L-asparaginase from an endophytic fungi of C.roseus such Nigrospora sp., Madurella sp. and Lasiodiploidia sp. Hence this property of producing such a novel enzyme by these endophytes can be exploited for the production of L-asparaginase which can be further used in pharmaceutical and food industries.
Authors and Affiliations
PUSHPA H, KAVYA S, POOJA K, SNEHA L, VARSHA O ARER
Keywords
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