An overview on application of phage display technique in immunological studies
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 7
Abstract
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and isolated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.
Authors and Affiliations
Abbas Rami, Mahdi Behdani, Fatemeh Kazemi-Lomedasht, Najmeh Yardehnavi
Keywords
Related Articles
- EP ID EP269185
- DOI 10.1016/j.apjtb.2017.06.001
- Views 71
- Downloads 0
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