AN OVERVIEW ON HIGH PERFORMANCE LIQUIDCHROMATOGRAPHY- MASS SPECTROSCOPY
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 5
Abstract
Ultra high performance liquid chromatography\ms is an extremely versatile instrumental technique. As the name suggested the instrumentation comprises a high performance liquid chromatography attached, via suitable interface, to a mass spectrometer. The primary advantage UHPLC/MS has over GC/MS is that it is capable of analyzing a much wider range of components. Compounds that are thermally liable, exhibit high polarity or have a high molecular mass may all be analyzed using UHPLC/MS even proteins may be routinely analyzed. Ultra high performance liquid chromatography (UHPLC) performs separations 5 to 10 times faster than conventional HPLC by employing sub-2 μm diameter particles. The 1-2 second peak widths and relatively high separation efficiency of UHPLC are more competitive with capillary GC, making UHPLC-MS an attractive alternative method for illicit drug analysis. The columns packed with porous sub-2μm particles and the extension of the upper pressure limit of HPLC instrumentation to 1300bar (ultra-high pressure liquid chromatography, UHPLC) has opened new frontiers in resolution and speed of analysis. However, certain constraints appear when coupling UHPLC technology with mass spectrometry (MS). Firs Chromatography (HPLC) directly coupled to mass spectrometry (MS) was in routine use in drug metabolism laboratories. It can give Enhanced selectivity and sensitivity, and rapid, generic analysis
Authors and Affiliations
Navya Sri D* , Ramamohan Reddy T1 , Ajitha A1 , Uma Maheshwara Rao. V1
Keywords
Related Articles
- EP ID EP101160
- DOI -
- Views 129
- Downloads 0
DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF PARACETAMOL AND DICLOFENAC SODIUM IN BULK AND TABLET DOSAGE FORM
Two simple, specific, sensitive, rapid, precise, accurate and economical UV Spectrophotometric methods viz. simultaneous equation method and Q-Absorbance ratio method have been developed and validated for the routine est...
DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF TRIFLUOPERAZINE IN BULK AND TABLET PHARMACEUTICAL FORMULATION
ABSTRACT The aim of present research work was to develop and validate UV spectroscopic method for estimation of Trifluoperazine in bulk and tablet pharmaceutical formulation. The method employed simple spectrosco...
SCREENING OF WOUND HEALING ACTIVITY OF BARK OF ALEURITES MOLUCCANA L.
Aleurites moluccana L. is used in folklore medicine in fomenting, sealing of secondary infection, healing of wounds and skin eruptions. There was no scientific evidence justifying the use of bark of Aleurites moluc...
PHYTOTOXICITY ANALYSIS OF DIFFERENT CARPET DYES AND THEIR FUNGAL EXTRACTED METABOLITES (FUNGAL DEGRADED PRODUCTS) THROUGH RELATIVE SEED GERMINATION AND SEEDLING GROWTH PARAMETERS
To assess the phytotoxicity of five different carpet dyes viz. Methyl Red, Methyl Orange, Erichrome Black, Crystal Violet and Malachite Green and their extracted metabolites, (degraded products) an experiment was s...
PHARMACOGNOSTICAL AND PHARMACEUTICAL ANALYSIS OF VASADI VATI IN THE MANAGEMENT OF TAMAKA SHWASA W.S.R. TO BRONCHIAL ASTHMA
Tamaka Shwasa is a Swatantra Vyadhi& having its own etiology, pathology & management. Its clinical feature resembles with the Bronchial asthma, a very distressing disease of respiratory system producing dyspnoea...