DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF TRIFLUOPERAZINE IN BULK AND TABLET PHARMACEUTICAL FORMULATION
Journal Title: Int J of Pharm Res & Analy - Year 2016, Vol 6, Issue 2
Abstract
ABSTRACT The aim of present research work was to develop and validate UV spectroscopic method for estimation of Trifluoperazine in bulk and tablet pharmaceutical formulation. The method employed simple spectroscopy based on the solubility of Trifluoperazine in water. For determination of wavelength different concentration samples were scanned in 200- 400 nm range and the maximum absorbance was found at 257 nm wavelength. Lambert–Beer’s law for trifluoperazine were followed in theconcentration range of 1-16 μg/ml (r2 = 0.9999) respectively. The method is validated as per ICH guidelines. Accuracy was assessed by the standard addition method. The recoveries were obtained in range of 98.03-101.64%. The repeatability was determined by RSD for Trifluoperazine and were found to be 0.58% respectively. The intraday precision and interday precision was determined by RSD and were found to be 0.54-0.77% and 0.28-1.00% respectively. The LOD and LOQ value for Trifluoperazine was found to be 0.29 and 0.89 µg/ml.The newly developed method can be used or routine analysis in laboratories and it is suitable for the quality control of the raw material, formulations, and dissolution studies. Keywords: Trifluoperazine, UV spectrophotometry, Method optimization, Validation.
Authors and Affiliations
Kanji V. Vaghela* , Ashok B. Patel, Ajay I. Patel, Nilesh K. Patel, Amit J. Vyas
Keywords
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