DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR PACLITAXEL IN BULK AND ITS PHARMACEUTICAL DOSAGE FORM
Journal Title: Int J of Pharm Res & Analy - Year 2013, Vol 3, Issue 2
Abstract
A simple, precise, rapid and accurate reverse phase HPLC method has been developed for the determination of Paclitaxel in bulk and its pharmaceutical dosage form. An enable C18G, 250mm, X4.6mm i.d, 5μm particle size column was used with photo diode array UV-Visible detector. The mobile phase consisting of Acetonitrile and water in the ratio of 80:20V/V was used. The flow rate was 1ml/min and the effluent was monitored at 228nm. The retention time of the drug was 3.737 minutes. The method was linear over the concentration range of 30-250μg/ml. the method precision for the determination of assay was below 2% RSD. The percentage recovery of paclitaxel was 99.41 – 100.83%. The validation of method was carried out utilizing ICH guidelines.
Authors and Affiliations
Sree Rama Murthy Pyla , Jogendra Kumar YVV , Jagadeesh Panda , Vara Prasad A, , N. Sriram
Keywords
Related Articles
- EP ID EP131936
- DOI -
- Views 105
- Downloads 0
DEVELOPMENT AND VALIDATION OF A RP- HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ENALAPRIL MALEATE AND RAMIPRIL IN BULK AND TABLET DOSAGE FORM
A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Enalapril maleate and Ramipril in bulk and tablet dosage form. Chr...
ASSAY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF PAROXETINE AND CLONAZEPAM BY RP- HPLC
A simple, precise, rapid, specific and accurate reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Paroxetine and Clonazepam in pharmaceutical dosage form. Chromatogr...
METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF EZETIMIBE AND GLIMEPIRIDE BY RP-HPLC
ABSTRACT A sensitive, selective and precise high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Ezetimibe and Glimepiride in tablet dosage form. T...
RP-HPLC METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF TRITHIOPARAMETHOXYPHENYL PROPANE (ANETHOLE TRITHIONE) AND CHLORPHENIRAMINE MALEATE IN TABLET DOSAGE FORM
ABSTRACT A Simple, precise and rapid RP-HPLC method was developed and validated for simultaneous Estimation of Trithiparamethoxyphenyl propene (Anetholetrithione) and Chlorpheniramine Maleate in tablet dosage form....
A REVIEW ON CURRENT TRENDS AND ADVANCEMENTS IN BIOANALYTICAL METHODS
This paper reviews the recent developments in bio-analysis sample preparation techniques and chromatographic techniques give an update on basic principles and a comparative discussion on the benefits and limitation of ev...