AN OVERVIEW ON HPLC, UPLC AND ITS TROUBLESHOOTING

Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4

Abstract

HPLC and UPLC Systems take advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. When taken together, these achievements have created a stepfunction improvement in chromatographic performance.Although HPLC method development has been improved by advances in column technology and instrumentation, problems still arise. This helps in systematic means of isolating, identifying, and correcting many typical problems. The important segments of an HPLC and UPLC system are the same, whether you use a modular system or a more sophisticated unit except the column characteristics. Problems affecting overall system performance can arise in each component. Solutions to these problems are presented in easy-to-use tables.

Authors and Affiliations

Nidhi Nk* , Neelam I , AjithaA , Uma Maheswar Rao V

Keywords

Related Articles

 DEVELOPMENT AND VALIDATION OF LORATADINE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV SPECTROSCOPIC METHOD

 A simple UV spectroscopic method was developed and validated for the estimation of loratadine in bulk and pharmaceutical dosage form using acetonitrile as solvent. The quantification was achieved at 250nm. Beers la...

 RECENT APPROCHES FOR IMPURITY PROFILING IN PHARMACEUTICAL FORMULATIONS

 In the pharmaceutical world, an impurity is considered as any other organic material, besides the drug substance, or ingredients, arise out of synthesis or unwanted chemicals that remains with API’s. The impurity m...

 ANALYTICAL TECHNIQUES FOR THE ESTIMATION OF MIRTAZAPINE IN BULK AND PHARMACEUTICAL DOSAGE FORMS: A REVIEW

 ABSTRACTMirtazapine (MRZ) has a piperazinoazepine structure {1,2,3,4,10,14b-hexahydro-2- methylpyrazino [2,1-a]-pyrido[2,3-c][2] benzazepine}. It belongs to the class of noradrenergic and specific serotonergic inhi...

FORMULATION AND EVALUATION OF FLOATING DRUG DELIVERY SYSTEM OF ZIDOVUDINE

Zidovudine is considered a good candidate for incorporation in a gastro-retentive dosage form due to its high solubility in the stomach medium compared with its solubility in the small intestine medium. As its solubility...

FINGERPRINT ANALYSIS OF HERBAL MEDICINES USING HPLC: AN OVERVIEW

Fingerprint analysis approach using HPLC has become the most potent tools for quality control of herbal medicines because of its simplicity and reliability. It can serve as a tool for identification, authentication and q...

Download PDF file
  • EP ID EP90307
  • DOI -
  • Views 183
  • Downloads 0

How To Cite

Nidhi Nk*, Neelam I, AjithaA, Uma Maheswar Rao V (2014). AN OVERVIEW ON HPLC, UPLC AND ITS TROUBLESHOOTING. Int J of Pharm Res & Analy, 4(4), 265-273. https://europub.co.uk/articles/-A-90307