Analysis of efficiency of the double-centrifugation protocol to prepare platelet rich<br /> plasma (PRP) – an experimental study in rabbits
Journal Title: RSBO - Year 2009, Vol 6, Issue 3
Abstract
Introduction and objective: The purpose of this study was to evaluaten the concentrations of platelets obtained from platelet rich plasma (PRP) prepared according to the double-centrifugation protocol. Material and methods: Eight adult male rabbits (White New Zealand) weighing 2. 8 to 4 kg were used. After general anesthesia, 10 ml of blood were drawn from each animal via cardiac puncture. Each blood sample was centrifuged according to the protocol of Sonnleitner et al. (2000). The peripheral blood (total) from each animal and the PRP samples platelets were counted manually. Data were submitted to statistical analysis. The normality of the data was confirmed and the Student’s t test was applied (p < 0.05). Results: PRP samples presented an average platelet count significantly higher than that of peripheral blood.Conclusion:Within the limits of this study, it was concluded that the double-centrifugation protocol was adequate to prepare PRP .
Authors and Affiliations
Michel MESSORA, Maria NAGATA, Flávia FURLANETO, Tatiana DELIBERADOR, Luiz MELO, Valdir GARCIA, Alvaro BOSCO
Keywords
Related Articles
- EP ID EP83525
- DOI -
- Views 245
- Downloads 0
Evaluation of the effect of use of occlusal splints and increase of vertical dimension of occlusion based on polysomnographic parameters in patients with obstructive sleep apnea syndrome
Introduction: Obstructive sleep apnea and hypopnea syndrome is the complete obstruction of the airways by the collapse of the oropharynx, soft palate and dorsal tongue for approximately 10 seconds, with a frequency...
Tratamento odontológico em pacientes com doença cardiovascular
Introduction: The correct evaluation of patients with cardiovascular disease is based on a careful anamnesis and on the previous knowledge of the main coronary diseases and its sequels. Objective: This article revised th...
Ethical and legal implications of marketing in Dentistry
Introduction and objective: The aim of this study was to discuss the ethical and legal use of marketing in dentistry by the professionals. Marketing itself is very important for solving the problems of competitiveness of...
Bond Força de auto-Utilização de Sistemas adesivos em esmalte Não moído e solo
The objective of this study was to evaluate the microshear bond strength of all-in-one self-etching adhesive systems on unground and ground enamel, and discuss some concepts involving these adhesives in enamel. Seventy e...
A Influência de Diferentes Métodos de Obtenção NA Ampliação do comprimento cervical de Trabalho em Primeiros testes Canais mésio-vestibular do molar superior
A ampliação dos canais radiculares utilizando diferentes tipos de métodos e instrumentos é o foco de inúmeras pesquisas na área de Deontologia. O objetivo deste estudo foi determinar a influência de quatro sistemas disti...