ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CARBIDOPA, LEVODOPA AND ENTACAPONE IN ITS BULK AND TABLET DOSAGE FORM BY UPLC
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 11
Abstract
The study was conducted with the purpose to method develop and validate a Ultra Performance Liquid Chromatography method UV detector to determine Levodopa, Carbidopa and Entacapone content in table preparation using MeOH : Acetonitrile : phosphate buffer (pH 2.8, 30:40:30 v/v/v). Column is BEH C18 (2.1 x 100 mm, 5 m), flow rate 0.3 ml per minute and run time is 4 minutes. The method was validated toward parameters of accuracy, precision, linearity, LOD, LOQ, flow rate, robustness of the Levodopa, Carbidopa, and Entacapone tablet in the form of LOD respectively 0.0060, 0.0064, and 0.35 and LOQ respectively 1.30, 1.44 and 1.96
Authors and Affiliations
Raja. Alagar , Ch. Shyamsunder, David Banji, K. N. V Rao. , Selva. Kumar
Keywords
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- EP ID EP104535
- DOI 10.7897/2230-8407.041113
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