ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF OMEPRAZOLE AND ASPIRIN USING REVERSE PHASE HPLC METHOD IN BULK AND DOSAGE FORM
Journal Title: Universal Journal of Pharmaceutical Research - Year 2017, Vol 2, Issue 4
Abstract
A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Aspirin and Omeprazole in bulk and pharmaceutical dosage form using C18 column (Agilent, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Methanol and 0.1 M Dipotassium Phosphate buffer (pH 3) in the ratio of 60:40 v/v. The detection was carried out at 256 nm. The method was linear over the concentration range for Omeprazole 50-250 μg/ml and for Aspirin 10-50 μg/ml. The recoveries of Omeprazole and Aspirin were found to be 100.07 and 100.06% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.
Authors and Affiliations
Vani R, Sunitha M
Keywords
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- EP ID EP36402
- DOI https://doi.org/10.22270/ujpr.v2i4.R6
- Views 354
- Downloads 0
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